2.3.5. Experimental Conditions

The assessment of mitochondrial respiration was performed after addition of carrier substance (distilled water)—control, or different concentrations of pravastatin (100 μM, 300 μM and 1000 μM). For experiments with gemfibrozil (100 μM, 300 μM and 1000 μM), dimethyl sulfoxide (DMSO) (Sigma-Aldrich Corporation, St. Louis, MO, USA) 0.5%, 1.5% and 5% respectively were used as controls. The incubation took place at room temperature (kept at 21 ◦C) for 3 min. Eight biological and three technical (three separate measurements from a single homogenate) replicates were performed.
