*4.3. Antibodies*

Rabbit polyclonal antibodies that were used included anti-PLIN5 (#26951-1-AP, Proteintech, Wuhan, China), anti-ATF1 (#11946-1-AP, Proteintech, Wuhan, China), anti-ATF4 (#10835-1-AP, Proteintech, Wuhan, China), anti-MAPK14 (p38; #A10832, Abclonal, Wuhan, China), anti-Phospho-MAPK14-T180/Y182 (#AP0526, Abclonal, Wuhan, China), anti-Phospho-JNK1/2/3- T183/T183/T221 (#AP0631, Abclonal, Wuhan, China), anti-ASK1 (#A6274; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti- p-ASK1 (#AP0394; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-VDAC1/Porin (#55259-1-AP, Proteintech, Wuhan, China), and anti-GAPDH (#AC027, Abclonal, Wuhan, China). The mouse monoclonal antibody that was used included anti-JNK1/2/3 (#A11119, Abclonal, Wuhan, China). The following secondary antibodies were used: HRP (horseradish peroxidase)-labeled Goat Anti-Rabbit IgG (H+L; #AS014, Abclonal, Wuhan, China), and HRP-labeled Goat Anti-Mouse IgG (H+L; #AS003, Abclonal, Wuhan, China).
