*2.11. Paraquat*/*Glutathione Assays*

Flies (2–3 days old) were starved for 2 h in 0.5% (*w*/*v*) agar and transferred into vials containing fiberglass filter papers with 5% (*w*/*v*) sucrose and 20 mM PQ with or without 220 μM glutathione (reduced). For the glutathione experiments, just eclosed flies were also reared on media containing 220 μM glutathione (reduced) for 1 week prior to PQ survival studies. Numbers of dead flies were counted every 12 h and plotted (n ≥ 20).

#### *2.12. Cloning of Human BKCa*/*UAS-Flies*

BKCa-HF full length was cloned in pUASTattb vector between NotI and XhoI restriction enzyme sites. The construct was amplified by PCR using N-terminal c-myc tag pCDNA3BKCa-HF as a template. Briefly, BKCa-HF was amplified using sense primer 5-AAG GAA AAA AGC GGC CGC ATG GGC GCC GAG GAG CAG AAG-3 and anti-sense primer 5-CTA GTC TAG ACT CGA GTC AAA GCC GCT CTT CCT G-3. The PCR conditions were 95 ◦C for 5 min, 30 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 7 min followed by extension at 72 ◦C for 10 min. The clones obtained were confirmed by sequencing (Genewiz). Constructs were injected into *Drosophila* embryos using services from BestGene Inc (Chino Hills, CA, USA).
