*4.17. Statistical Analyses*

All quantitative experiments were evaluated for statistical significance using the software GraphPad Prism v.5.0 (GraphPad Software, Inc. 7825 Fay Avenue, Suite 230 La Jolla, CA, USA), after verifying the normality of values and equivalence of variances. For lipid droplet counts, pixel quantification, LD-mitochondria contact site counts, fluorescence intensity, and qPCR analyses, means ± s.d. are displayed, and the statistical di fferences between overexpression or RNAi-treated or peroxide hydrogen-treated samples and controls were addressed using Student's two-tailed *t*-tests. The Student's *t*-test was utilized because the sample size in the experiment was small, and a sample mean and standard deviation can be obtained, and additionally samples came from normal or approximate normal population. A *p*-value < 0.05 was considered statistically significant.

**Author Contributions:** Y.J. conceived and designed the experiments. Y.T. and Q.W. performed the experiments (Y.T. performed the main part). Y.T. and Q.W. analyzed data. J.H. and X.W. contributed to the preparation of reagents/materials/analysis tools. Y.T. wrote the manuscript. Z.R. supervised this study.

**Funding:** This work was supported by the Fundamental Research Funds for the Central Universities (No. 2662018PY043), Hubei Agricultural Sciences and Technology Innovation Center Team of Livestock and Poultry Genetic Improvement and Healthy Breeding (2019-620-000-001-30) and the National Project for Breeding of Transgenic Pig (No. 2016ZX08006-002).

**Conflicts of Interest:** The authors declare that they have no competing interests.
