*2.1. Animals*

Three-month-old male adult WT (129S1/SvImJ) and SIRT3−/− (Sirt3tm1.1Fwa) mice (20–25 g) were purchased from Jackson Laboratory (Bar Harbor, ME, USA). All experiments were performed according to protocols approved by the UPR Medical Sciences Campus Animal Care and Use Committee and conformed to the National Research Council Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (2011, eighth edition).
