**3. Conclusions**

Analytical performances and fitness for purpose of two commercial immunoassays widely applied for the detection of AFM1 in milk (strip test and ELISA) were evaluated, according to guidelines set in Regulation 519/2014/EU. Both assays showed satisfactory performances in terms of precision, recovery rates, false positive and false negative rates. In addition, the method performance profiles of the two methods obtained in the validation study could be verified by long-term intra-laboratory QC data. A good correlation between the results provided by the validated assays and the AOAC reference method was observed when analyzing naturally contaminated samples. The extension of the scope of the strip test method to goa<sup>t</sup> and sheep milk was successfully evaluated by applying the experimental design foreseen in the EU regulation.

#### **4. Materials and Methods**
