*3.3. Assessment of the OSSS after Provocation*

Seven of nine participants showed lower OSSS at week 24 compared to baseline (Figure 2A) demonstrating a change in the OSSS after provocation. The change from mean 11.7 at baseline to mean 7.3 at week 24 was not significant (*p* = 0.098). The change in the OSSS over the course of the study was also assessed (Figure 2B–D). There was a significant reduction in the mean OSSS from week 8 to 24 (mean OSSS ranging from 6.4–9.1) compared to baseline (mean OSSS 11.7) (Figure 2B). The reduction in the OSSS in seven of nine participants over the study period compared to baseline was statistically significant (*p* = 0.03) (Figure 2C,D), demonstrating a sustained reduction in organ-specific symptoms after vaccination of the cat observed from week 8 to week 24.

**Figure 2.** Organ specific symptom score (OSSS) after provocation. (**A**) Individual OSSS with SEM of participants (*n* = 9) at baseline versus week 24 at the end of the study. (**B**) Mean OSSS with SEM of participants (*n* = 9) at baseline and over the course of the study. (**C**) Individual mean OSSS with SEM comparing baseline (weeks 1–3, *n* = 3) vs. treatment period (weeks 8, 12, 16, 20 and 24, *n* = 5). (**D**) Individual mean OSSS (*n* = 9) with SEM comparing baseline (*n* = 3, weeks 1–3) vs. treatment period (*n* = 5, weeks 8, 12, 16, 20 and 24). Statistical significances were obtained by an exact Wilcoxon matched-paired signed rank test. Possible OSSS values from 0–30.
