4.4.1. Circular Dichroism Spectra

The CD spectra were recorded in the ultraviolet (UV) region of 190 to 250 nm with Chirascan plus CD spectrometers (Applied Photophysics Ltd., Leatherhead, UK), equipped with an optional Peltier temperature controller for rapid and precise temperature control of the sample cell (Quantum North West, 22910 E Appleway Avenue, Suite 4 Liberty Lake, WA, USA), in 0.01 M sodium phosphate buffer (pH 7.0) and 20 ◦C. The average molecular weight of the amino acid residue for calculation of molar ellipticity [ ] (degree cm<sup>2</sup> dmol−1) was assumed to be 112 Da. The secondary structure elements were calculated by the Provencher–Glöcker method CONTIN/LL modified by Sreerama N. of the CDPro software package, 2000 (Colorado State University, Fort Collins, Colorado, USA, http://lamar.colostate.edu/sreeram/CDPro) [59,60]
