*4.6. Liquid Chromatography/Ion Trap Mass Spectrometry*

The sample, column, mobile phase, and gradient conditions were the same as those used for the HPLC analysis (12). The LC separation was performed using the Agilent 1100 HPLC system (Agilent Technologies, Palo Alto, CA, USA). Five microliters of the sample was filtrated using an Ultrafree-MC membrane centrifuge filtration unit (hydrophilic PTFE, 0.20 μm, Millipore, Bedford, MA, USA) and loaded onto a TSK-gel Super ODS column (2.0 μm, 2.0 × 100 mm, TOSOH, Tokyo, Japan) at 40 ◦C. The mobile phase was 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). The flow rate was 200 μL/min with UV detection at 254 nm. The gradient conditions were initially 10–90% B for 40 min. The entire eluate was directed into the mass spectrometer, where it was diverted to waste 2.5 min after injection to avoid any introduction of salts into the ion source. The MS analysis was accomplished using a Finnigan LCQ Deca XP plus ITMS (Thermo Fischer Scientific, San Jose, CA, USA) equipped with an electrospray ionization (ESI) interface. The ESI conditions in the positive ion mode were as follows: capillary temperature 300 ◦C, sheath gas flow rate 35 (arbitrary unit), ESI source voltage 5000 V, capillary voltage 43 V, and tube lens offset 15 V. Various scan ranges were used according to the molecular weights of the tested compounds.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1660-3397/16/2/50/s1, Figure S1: Total ion chromatograms (a) and (b) and selected ion monitoring (SIM) at *m*/*z* 722.4 (c) and *m*/*z* 564.3 (d) of fumonisin B1 and a reaction product by microbial degradation using B-9 at 0 h (A) and 96 h (B), respectively, Figure S2: (A) HPLC chromatograms of MC-LR by B-9 without EDTA after 0, 6 and 24 h. (B) HPLC chromatograms of MC-LR by B-9 with EDTA after 0, 6 and 72 h.

**Acknowledgments:** K.-I.H. and H.J. gratefully acknowledge Tatsuko Sakai at the Analytical Services Center, Faculty of Pharmacy, Meijo University, for assistance and support.

**Author Contributions:** K.-I.H. and H.J. conceptualized the research. K.T., H.U. and Y.O. performed the degradation experiments of drugs including antibiotics with the supervision of K.-I.H.; E.H.H. and M.K. performed the experiments of mycotoxin degradation with the guidance of K.-I.H.; Y.H. and H.J. performed degradation of amide and esters with the guidance of K.-I.H.; M.K., Y.H. and H.J. ran the LC/MS and analyzed the LC/MS data under the supervision of A.R.J.A.; H.J. and K.-I.H. wrote the manuscript. All co-authors agreed to the contents of the paper.

**Conflicts of Interest:** The authors declare no conflict of interest.
