4.6.2. Effects of Temperature on Activity and Stability of Cadex

The temperature for the enzymatic reaction was optimized by experimentation at different temperatures (0–70 ◦C). To assess thermal stability, the enzyme solution was pre-heated at temperatures of 30 ◦C, 40 ◦C, and 50 ◦C for 0–5 h. Residual enzyme activity was measured at each interval.
