4.4.2. UV Absorption Spectra

Absorption spectra of proteins were recorded with a UV-Visible spectrophotometer UV-1601 PC (Shimadzu, Kyoto, Japan) in quartz cells with an optical path length of 1 cm, 0.1 cm, and 0.01 cm in the range of 190 to 400 nm. The molar extinction coefficient of enzyme ε<sup>280</sup> = 100,770 M−<sup>1</sup> cm−<sup>1</sup> was calculated from the content of aromatic amino acids using the ExPASy server [61].
