*4.12. SDS-PAGE*

SDS-PAGE was performed by the method of Porzio and Pearson [53] using 10% polyacrylamide gel. Proteins in the gel were stained with 0.1% (*w*/*v*) Coomassie Brilliant Blue R-250–50% (*v*/*v*) methanol–10% (*v*/*v*) acetic acid and the background of the gel was destained with 5% (*v*/*v*) methanol–7% (*v*/*v*) acetic acid.
