**4. Conclusions**

In conclusion, we purified and characterized a new alginate lyase, Aly08, from marine bacterium *Vibrio* sp. SY01. Its special characteristics (such as: thermo-tolerance and pH stability) make Aly08 a superior candidate for industrial applications. Further analysis will focus on analyzing the three-dimensional structure of Aly08 and exploring its molecular mechanisms.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1660-3397/17/5/308/s1, **Figure S1.** Viscosity measurement of Aly08; **Table S1.** Primers used in this study.

**Author Contributions:** S.L. and Y.H. designed the experiments. Y.W., L.B., Y.R., Q.H. and Z.Y. conducted the experiments. S.L., X.C. and R.Y. analyzed the data. Y.W., S.L. and Y.H. wrote the main manuscript. All authors reviewed the manuscript.

**Funding:** This research was funded by Shandong Provincial Natural Science Foundation grant number ZR2019BD027; National Nature Science Foundation of China, grant numbers 81573451 and 81602621; the Starting Research Funding of Qingdao University, grant number 41118010106 and 41118010250; the Key Lab of Marine Bioactive Substance and Modern Analytical Techniques, SOA, grant number MBSMAT-2018-06; and the Key Research and Development Program of Shandong Province, grant number 2018YYSP024.

**Conflicts of Interest:** The authors state that there is no conflict of interest.
