*4.3. Degradation of Tested Compounds*

Two milligrams of the tested compounds was dissolved in 1 mL of EtOH and 50 μL of the solution was evaporated to dryness. One milliliter of the preincubated cell broth of strain B-9 (containing approximately 3 × 106 colony forming units (CFU) mL−1) was added to the residue. The resulting solution was mixed, and then incubated at 27 ◦C for 5, 15, 30, 60, and 120 min. After incubation, 50 μL of each of these mixtures was added to 50 μL of MeOH containing 0.2% FA and filtered using an Ultrafree-MC membrane centrifuge-filtration unit (hydrophilic polytetrafluoroethylene (PTFA), 0.20 μm, Millipore, Bedford, MA, USA) to stop the degradation and to eliminate proteins. Each supernatant was then analyzed by HPLC and LC/MS.

#### *4.4. Enzyme Inhibition*

Enzyme inhibitors were prepared as follows: EDTA was prepared as a 200-mM stock solution in water and was used at an assay concentration of 10 mM. PMSF was prepared as a 200-mM stock solution in EtOH and was used at an assay concentration of 10 mM. The cell broth and required inhibitor were preincubated at 27 ◦C for 30 min.
