*2.12. Statistical Analysis*

The results were reported as means ± standard deviations (SD) performed in triplicate. A *t*-test was used to assess the differences in antibacterial activity. Significant differences among the data were estimated by applying analysis of variance (ANOVA). The Tukey's least significant differences (LSD) test was used to evaluate the significance of the analysis. Differences were considered significant at *p* < 0.05. All statistical tests were performed with IBM SPSS Statistics for Windows, Version 25.0 (IBM Corp., Armonk, NY, USA).
