*2.5. Visualizing Co-Culture Biofilm Following Desiccation Treatment Using Scanning Electron Microscopy (SEM)*

The co-culture and mono-culture samples were generated as described above. One milliliter of each sample was harvested by centrifugation (at 4000× *g* for 10 min) and washed once with DW. Then, 5 μL of suspension from each sample was placed on polylysine-coated glass slides and left open in the dry cabinet at 40% relative humidity and at 25 ◦C for 20 h. Before analysis in the SEM, the slides were coated by gold/palladium coating (20:80), at 12 mA voltage and 1 nm thickness.
