*2.10. E*ff*ect of Cell-Free Culture Supernatant on S. aureus Growth*

The, *S. aureus* starter culture was prepared into 10 mL of TSB medium overnight at 37 ◦C and 150 rpm. Then, 150 μL of the generated bacterial suspension was introduced into 15 mL of fresh TSB medium. For the antibacterial test, the *S. aureus* suspension was supplemented by the cell-free supernatant prepared as described above. The samples were incubated for 8 h at 37 ◦C at 150 rpm and subjected to OD600 measurements every 1 h.
