*3.6. Immunofluorescence Staining*

Cells (2000 cells/well) were allowed to seed in a 96-well plate (CellCarrier, Perkin Elmer, Shelton, CT, USA, part number: 6,005,558) containing 0.4 μL of a compound at room temperature for 30 min and then placed under 5% CO2 in a humidified environment at 37 ◦C. After 72 h incubation, 100 μL of a solution containing 1/1000 Hoechst 33,342 (Molecular Probes, Invitrogen, Carlsbad, CA, USA), 1 μM MitoTracker Deep Red (Molecular Probes, Invitrogen, Carlsbad, CA, USA) and 1/1000 CellMask Plasma Membrane (Molecular Probes, Invitrogen, Carlsbad, CA, USA) were added to each well and incubated for 30 min at 37 ◦C. Stained cells were washed once with PBS to remove excess stains and then replaced with 100 μL of PBS before being imaged.
