*2.2. Analysis of CRISPR*/*Cas9-Induced Mutations in GLA Gene*

To identify the presence of indels in *GLA* gene, the genomic DNA was extracted and used for PCR amplification of the target site with the primer pair 5 -CACACACCAACCTCTAACGATACC-3 (forward) and 5 -CCAGGAAAGGTCACACAGAGAAAG-3 (reverse). PCR products were TA-cloned into pGEM-T Easy vector (Promega, Madison, WI, USA). Subsequently, DNA from the clones #19 and #27 was sequenced using T7 forward and Sp6 Reverse primer. Vector NTI software was used to align the results of sequencing and determine the indel spectra in *GLA* target site.
