*2.6. Mathematical Modelling and Computer Simulations*

To simulate the BKCa current in silico, we extended a previously published formulation [22] to include (1) calcium dependence according to the Lin et al. [23] data, and (2) inactivation kinetics according to Ding et al. [24] data. To demonstrate the contribution of BKCa current on AP repolarization, we integrated the BKCa model to a well-established human left ventricular CM model [25].

#### *2.7. Statistical Analysis*

Results are presented as mean values ± SEM. Area under the curve was calculated by using the GraphPad Prism Software 5.02 (GraphPad Software, San Diego, CA, USA). Statistical differences were evaluated by using the Student's *t*-test (paired or unpaired) or repeated measures ANOVA, followed by a Bonferroni test, where appropriate. A value of *p* < 0.05 was considered to be statistically significant.
