*4.11. Statistical Analysis*

Experimental data were analyzed by GraphPad Prism. Student's *t*-test was performed to compare two groups. Data are expressed as means = +/− SEM; \*\* *p* < 0.001.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-0817/9/5/372/s1, Figure S1: Schematic for synthesis of Alendronate conjugation of DSPE-PEG(2000) from DSPE-PEG(2000) Carboxylic Acid and alendronate sodium trihydrate.

**Ethical Statement:** All experiments related to virulent strain of *Mycobacterium tuberculosis* were carried out inside the BSL3 facility. All animal experiments were conducted in line with "The Indian Animal Ethical Committee" regulations.

**Author Contributions:**All the authors have contributed according to their expertisein this study. J.G.: Conceptualization, methodology, validation, formal analysis, investigation, visualization, project administration, Original Draft, writing—reviewing and editing. S.M.: Validation, data curation, writing—reviewing and editing. V.R.: Methodology, writing—reviewing and editing. R.B.: Supervision, project administration, funding acquisition. All authors have read and agreed to the published version of the manuscript.

**Funding:** We extend our sincere thanks to the Department of Biotechnology, Government of India for financial support, specifically for the maintenance and upgradation of BSL-3 and ABSL-3.

**Acknowledgments:** We are grateful to DBT for providing financial assistance for the BSl-3 work. We would like to thank Gajender Saini (TEM) and A. K. Sahu (Confocal Microscope), Advanced Instrumental Research Facility (AIRF), JNU We also would like to thank Shashank Taxak (confocal image analysis).

**Conflicts of Interest:** The authors report no conflicts of interest in this work.
