*2.4. Reverse Transcription*

cDNA was synthesized according to the manufacturer's instructions of the iScript ™ cDNA Synthesis Kit (Bio-Rad). In the first stage of the reverse transcription reaction, 1 μg matrix RNA previously tested for quality and integrity was transferred from an isolated sample to 0.2 μL Eppendorf tubes, followed by the addition of reagents that are necessary for the cDNA synthesis process according to the manufacturer's proportions, which results in a mixture with a final volume of 20 μL. The reverse transcription reaction was carried out in a Biometra UNO II thermal cycler under conditions following the manufacturer's guidelines. The resulting cDNA was stored at −20 ◦C until analyzed.
