**4. Conclusions**

Di fferent bioinformatics tools were applied in this study to identify vital drug targets of *Mycobacterium avium* subsp. *hominissuis*. Protein sequences of *M. avium* subsp. *hominissuis* were parsed using multiple steps of the subtractive genomics approach, and a few of them were shortlisted as possible drug targets because they fulfilled the druggability criteria. The shortlisted sequences were non-homologous to the human host; thus, these can be proposed as ideal drug targets. All the identified drug targets of di fferent strains of MAH have never been characterized before as drug targets, and we proposed them here as potential drug targets against which new drug compounds can be designed. Therefore, the study is significant to the scientific community, as it provides a prioritized list of possible drug targets sorted by the computational subtractive genomics method, and it has the potential to lead to the discovery of new and novel drug targets against *M. avium* subsp. *hominissuis*.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-0817/9/5/368/s1, Table S1; Table S2; Table S3; Table S4; Table S5.

**Author Contributions:** Conceptualization, R.U. and A.A.-H.; methodology, BS.; software, M.R.; formal analysis, B.S.; investigation, R.U and A.K.; resources, R.U.; data curation, S.A.H.; writing—original draft preparation, R.U.; writing—review and editing, A.K.; visualization, A.K. and S.A.H.; supervision, A.A.-H.; funding acquisition, A.A.-H. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by Pakistan Science Foundation Grant# PSF-TUBITAK/S-HEJ (04) and the APC was funded by University of Nizwa.

**Acknowledgments:** The authors would gratefully like to acknowledge the financial support provided by the Pakistan Science Foundation Grant# PSF-TUBITAK/S-HEJ (04) for this project.

**Conflicts of Interest:** The authors declare no conflicts of interest.
