*4.4. Ethical Considerations*

This work received ethical approval from The University of Botswana IRB and the Human Research Development Committee (IRB of the Botswana Ministry of Health and Wellness, No: HPDME 13/18/1). All participants provided written informed consent.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-0817/9/3/180/s1, Figure S1: Gating Strategy, Figure S2: COMPASS analysis of Gag and Mtb-Lysate-stimulated CD8 and CD4 T-cells, Figure S3: Comparison of TNFα expression within total cytokine+CD4 T-cells, Figure S4: Comparison of activation profile of Gag-stimulated total cytokine+CD8+T-cells, Figure S5: Comparison of memory phenotypes between Mtb and HIV specific total cytokine+CD4+T-cells, Table S1: Antibody Panel.

**Author Contributions:** Conceptualization, S.G. and N.Z.; Methodology, T.R., C.A.M.M., E.N., T.J.S., and L.M.; Validation, C.A.M.M., L.M., E.N., and T.J.S.; Formal analysis, L.M.; Investigation, L.M.; Resources, S.G., S.S.S., T.J.S., and N.Z.; Data curation, L.M.; Writing—original draft preparation, L.M.; Writing—review and editing, T.R., S.M., I.K., T.M., S.G., S.S.S., E.N., and T.J.S.; Supervision, S.G., S.M., I.K., T.J.S., and E.N.; Project administration, S.G. and R.M.M.; Funding acquisition, S.G. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by the sub-Saharan African Network for TB/HIV Research Excellence (SANTHE), a DELTAS Africa Initiative (grant # DEL-15-006 to L.M., S.M., T.M., and S.G.), the Fogarty International Center, National Institute of Mental Health and National Institute of Allergy and Infectious Diseases of the National Institutes of Health (grant # D43 TW010543 to S.M. and gran<sup>t</sup> # K01AI118559 to SSS). The DELTAS Africa Initiative is an independent funding scheme of the African Academy of Sciences (AAS)'s Alliance for Accelerating Excellence in Science in Africa (AESA) and supported by the New Partnership for Africa's Development Planning and Coordinating Agency (NEPAD Agency) with funding from the Wellcome Trust (grant #107752/Z/15/Z) and the governmen<sup>t</sup> of the United Kingdom. R.M. and T.M. were partially supported by the EDCTP2 program Trials of Excellence in Southern Africa (TESA II), supported under Horizon 2020, the European Union. The views expressed in this publication are those of the authors and not necessarily those of the AAS, AESA, NEPAD Agency, Wellcome Trust, National Institutes of Health, or the UK government. The funders had no role in the study design, data collection, and decision to publish or in the preparation of the manuscript.

**Acknowledgments:** We wish to thank One Dintwe from the Cape Town HVTN Immunology Laboratory (CHIL) for the kind donation of the HIV Gag peptides used for the intracellular cytokine-staining assay.

**Conflicts of Interest:** The authors declare no conflicts of interest.
