*4.6. Statistical Methods*

Analysis of data was conducted using GraphPad Prism version 8.0.1 for Windows (GraphPad Software, La Jolla, CA, USA, www.graphpad.com). Statistical analysis between groups was performed using the analysis of variance (ANOVA) test. Survival data were analyzed using the Gehan–Breslow–Wilcoxon test. The growth index and *C*max were compared amongs<sup>t</sup> the H37Rv and Rostov strains using the unpaired t-test at each time point. A value of *p* < 0.05 was considered significant.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-0817/9/5/335/s1, Figure S1. MIRU-VNTRplus cluster analysis of *M. tuberculosis* Rostov strain, Table S1. Sublineage-specific nsSNPs in the virulence genes.

**Author Contributions:** Conceptualization, M.V.F. and E.A.S.; methodology, V.D.P., and N.K.F.; software, A.G.B., A.A.K. and J.A.B.; validation, L.V.D., T.I.K. and T.I.R.; formal analysis, A.A.K. and N.K.F.; investigation, N.S.G., T.I.K., T.I.R. and E.A.G.; resources, I.A.D. and V.D.P.; data curation, A.G.B.; writing—original draft preparation, M.V.F., E.A.S. and J.A.B.; writing—review and editing N.K.F.; visualization, E.A.G., and M.V.F.; supervision, V.D.P. and I.A.D.; project administration, V.D.P.; funding acquisition, I.A.D. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by a gran<sup>t</sup> from the Ministry of Science and Higher Education of the Russian Federation No. 075-15-2019-1671 (agreement dated 31 October 2019).

**Acknowledgments:** The authors are thankful to Khramov, M.V. and Sectoral Scientific Program of the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing.

**Conflicts of Interest:** The authors declare no competing financial interest.
