**Su-Jin Pyo 1, Deok-Gyeong Kang 1, Chuleui Jung <sup>2</sup> and Ho-Yong Sohn 1,\***


Received: 28 February 2020; Accepted: 17 March 2020; Published: 1 April 2020

**Abstract:** In Korea, various insect species such as crickets and grasshoppers, as well as honey bee and silkworm pupae, have been consumed as food and used in oriental medicine. In this study to evaluate useful the bioactivities and potentially adverse effects of edible insects, ethanol extracts of *Allomyrina dichotoma* (AD), *Tenebrio molitor* (TM), *Protaetia brevitarsis* (PB), *Gryllus bimaculatus* (GB), *Teleogryllus emma* (TE), and *Apis mellifera* (AM) were prepared and evaluated with regard to their anti-thrombosis, anti-oxidant and haemolysis activities against human red blood cells. AD and TE extracts showed strong anti-oxidant activities, which were not related to polyphenol content. All ethanol extracts, except AM extract, showed strong platelet aggregation activities. The platelet aggregation ratios of the extracts were 194%–246% of those of the solvent controls. The effects of the AD, TM, PB, GM, and AM extracts on thrombin, prothrombin and various coagulation factors were negligible. Only the extract of TM showed concentration-dependent anti-coagulation activities, with a 1.75-fold aPTT (activated Partial Thromboplastin Time) extension at 5 mg/mL. Of the six insect extracts, TM and AM extracts exhibited potent haemolytic activity. Our results on the insect extracts' functional properties suggest that edible insects have considerable potential not just as a food source but as a novel bio-resource as well.

**Keywords:** edible insect; blood coagulation; platelet aggregation; haemolysis; *Teleogryllus emma*
