*4.7. RNA Analyses*

Total RNA was isolated from leaf blades of the 4th leaves of rice seedlings using the Trizol reagent (Invitrogen, Carlsbad, CA, USA). Quantitative RT-PCR (qRT-PCR) was performed on a Thermal Cycler Dice TP800 system (Takara Bio, Tokyo, Japan) using SYBR premix Ex Taq mixture (Takara Bio) as previously described [5]. The primer sequences used for qRT-PCR are listed in Table 1.
