*4.6. Exogenous ABA Treatment*

A seed germination assay was performed as described above. For the ABA sensitivity assay, after-ripened seeds of NIL-NIP and NIL-ZS97 were treated with a series of ABA solutions (0, 1, 5, 10, 20, 50, and 100 μM). Dimethyl sulfoxide (DMSO) was used to dissolve ABA.

Subsequently, a 20 μM portion of ABA was chosen to treat after-ripened NIL-NIP and NIL-ZS97 to further measure the candidate gene expression. DMSO was used as control (CK). Embryos germinated for three days were used for RNA isolation.
