*4.7. Measurements of Detoxification-related Enzymes*

In order to determine the activity of GST, 0.3 g of leaf tissue was homogenized with 2 mL phosphate buffer solution (pH 6.5) + 1 mM EDTA. The suspension was centrifuged at 4000× *g* for 10 min. The GST activity was measured spectrophotometry at 412 nm following the method of Chun-hua and Ying [97]. The chitinase activity was measured according to the method of Chun-hua and Ying [97].
