*4.11. Experimental Design and Statistical Analysis*

The treatments were applied using a Completely Randomized Block Design (CRBD) with a factorial arrangement. All the obtained values are the means of three replicates ± standard deviation (SD). The data were analyzed using two-way analysis of variance (ANOVA) by SPSS v16.0 (SPSS, Inc., Chicago, IL, USA), and means were separated using Duncan's multiple range tests (α = 0.05).
