*2.3. Quality of RNA-Seq Data*

To investigate the transcriptional profiles of heat stressed and unstressed anthers of SDWG005, 15 RNA samples representing four different time points (1, 2, 6, and 12 h) under heat treatment (38 ◦C) and one control (28 ◦C) were sequenced individually with Illumina technology; the generated reads were used to assemble the transcriptome for each sample. Approximately 7.17 Gb of data (at least 20 million clean reads, Q30 > 93.2%) were obtained for each sample. The clean reads were used to assemble the transcriptome for each sample by mapping the reads to rice reference genome (MSU7.0). More than 90.0% of the reads could be mapped to the rice reference genome, and approximately 92% and 2% of the mapped reads were mapped to exon and intron regions, respectively. The mapped reads from all samples were then remapped to the reference genome; 1629 new genes/transcripts were discovered.
