*4.7. Statistical Analysis*

The data of two groups were analyzed by paired sample Student's test (*t*-test) and the data of more than two groups were analyzed by Tukey's test of one-way analysis of variance (ANOVA). Statistically significant differences at *P* < 0.05 between samples were indicated by different letters on the histograms or after mean values. All statistical evaluations were conducted using the IBM SPSS Statistics version 23 software (SPSS Inc., Chicago, IL, USA).
