4.3.1. TBI Model

A Cortical Contusion Injury device, which was purchased from Custom Design and Fabrication, Inc., (model eCCI-6.3), was used to simulate TBI. The mice were anesthetized with 3% isoflurane during the procedure. In order to induce TBI, a 3 mm rod impacted the scalp over the right hippocampal region at the velocity of 4 m/second and depth of 0.5 mm. This was repeated five times, with two days between each impact. Animals in the control and AG groups also underwent isoflurane anesthesia, but no impact.
