*2.11. Determination of Cell Apoptosis by Flow Cytometry*

The apoptotic cells were measured using the FITC-Annexin V Apoptosis Detection Kit. After drug treatment as a cell viability experiment, cells were harvested and then washed with 1 mL PBS. Next, cells were resuspended in 195 μL FITC-Annexin V binding buffer, and then 5 μL of FITC-Annexin V and 10 μL propidium iodide (PI) incubation buffer were added according to the manufacturer's instructions. The cells were incubated for 15 min at room temperature and protected from light. Samples were not stored, but analyzed immediately. Samples were analyzed with an imaging flow cytometer.
