*2.3. Water T Maze Test*

Hippocampal-dependent spatial memory was tested using a water T maze [24,25] according to our previous report [20]. The maze consisted of a start box, a left arm, and a right arm, which was filled with water at 23 ± 1 ◦C up to 1 cm above the surface of the platform. Mice were allowed to swim to the right or left arm. This screening step was repeated three times at 15 s intervals. The platform was placed on the side that the mice reached less often. After the screening step, mice were allowed to explore the maze freely. If mice reached the platform, they were allowed to rest there for 5 s (correct choice). If not, the arm entry was closed with a board and they were forced to swim for 15 s as a deterrent (incorrect choice). This trial step was repeated five times at 4-min intervals. Mice were subjected to this trial step for five days. To evaluate the results of the trial, the percentage of correct responses per day was determined. After two days of rest, to test hippocampal and prefrontal cortex (PFC)-dependent working memory, the platform was moved to the opposite arm. Similarly, mice were allowed to explore the maze freely. The trial step was repeated 15 times at 4-min intervals in a day. To evaluate the results of the trial, the percentage of the correct responses were calculated after every three responses.
