*2.12. Data Analysis*

Analyses of qPCR data utilized BioRad CFX Manager software (BioRad); one-way ANOVA followed by Bonferroni's test with the correction for multiple comparisons was used, with *p* < 0.05 set as criterion of statistical significance. Feeding data from studies comparing two groups were analyzed using a *t*-test, whereas comparisons between three or more groups were done with ANOVA followed by Bonferroni's post-hoc test, with differences considered significant when *p* < 0.05.
