*2.10. Y-Maze Test*

The apparatus used for the evaluation of the Y-maze was made of black-painted wood, and the dimensions of the arms were 50 cm long and 20 cm height and 10 cm width, as already used by our group [32] and others [33]. The mice were allowed to move freely in the Y-maze, and for that, the mice were kept in the center of the apparatus for 8 min, three times. The arm entries were visualized and observed carefully. The successive entry of the mice into the arms was defined as spontaneous alternations. The alternation behaviors were considered as (successive triplet sets (entries into three different arms consecutively)/total number of arm entries (2)) × 100. A higher percentage (%) of spontaneous alternations was considered to be an indicator of the improved cognitive performance of the experimental mice, and vice versa.
