*3.5. Chronic Administration of Curcumin Rescued the Neuronal Cell Loss, as Assessed by Fluoro-Jade B and Nissl staining*

Overall, the neuroprotective effects of curcumin were further confirmed by visualizing the morphology of hippocampal neurons, by using Nissl and FJB staining on the mice brains, as these staining protocols have extensively been used to visualize the morphology of neurons in different experimental settings [41,42]. According to FJB staining, there were increased FJB stained neurons in the ethanol-treated group, which was significantly inhibited with the administration of curcumin, as shown (Figure 5A). Similarly, in the Nissl staining, there was a decrease in the Nissl stained neurons in the ethanol injected group, which was significantly preserved with the administration of curcumin, as shown (Figure 5B), thereby confirming the hypothesis that curcumin plays a rescuing role against ethanol-induced neurodegeneration and memory impairment.
