4.3.2. CMS Model

The stress procedure lasted for six weeks. The mice were randomly subjected to two types of stressors each day. The potential stressors included 2 h of immobilization, strobe light exposure, white noise exposure, cat urine exposure, and overnight food deprivation, water deprivation, or light exposure. During the weekend, the mice were exposed to wet bedding or a tilted cage for 24 h. The control mice were left undisturbed in their home cage.

## *4.4. Behavioral Tests*

#### 4.4.1. Sucrose Preference

Anhedonia-like changes in the CMS mice were monitored using the sucrose preference test, which was performed at the 2nd, 4th, and 6th week. Consumption of water and sucrose by a single caged mouse was measured from 6 p.m. to 9 a.m. The preference index was calculated as follows: [sucrose consumption/(DW consumption + sucrose consumption)].
