*2.4. Open Field Test*

An open field test (OFT) was conducted 24 h after the 21 days of CORT treatment. An open box (60 × 60 × 50 cm) was used in the OFT. At the beginning of the test period, mice were placed in the center of the box to adapt for 3 min. The experimental parameters were set, and the test time was set to 10 min. The locomotor activity (movement distance, movement speed, and other indicators) was monitored and traced with an automated video-tracking system. Between one mouse and the next one, the apparatus was thoroughly cleaned with 75% ethanol, followed by distilled water and dried before using.
