*2.6. Episodic Intake of Individually Presented GM and CM in Sated Adolescent and Aged Rodents*

Mice and rats aged 5–6 weeks (*n* = 9–11/group for each species) were used in the study on adolescent animals, whereas 25-month old mice and 26-month old rats (*n* = 8–9/group for each species) were used as the aged cohorts. The feeding experiments utilizing individually presented cornstarch, sucrose, GM, and CM solutions followed the protocol described above for the relevant studies in adult sated rodents that received one of the four solutions for 2 h. This feeding experiment was conducted in a separate cohort of animals.

## *2.7. Episodic Intake of GM and CM Presented Simultaneously in Sated Adolescent, Adult, and Aged Rodents*

Mice (*n* = 20) and rats (*n* = 21) aged 5–6 weeks were used in the study on adolescent animals, 16–18-week old mice (*n* = 10) and rats (*n* = 12) were included in the study on adults, whereas 25-month old mice (*n* = 12) and 26-month old rats (*n* = 11) were used as the aged cohorts. Adult and adolescent rats and mice had been previously exposed to GM and CM (pre-exposure to both milk types was simultaneous). The aged animals came from the cohorts described above (pt. 2.6), however, a week-long 'washout' period was allowed between the previous experiment and this study. First, the animals were accustomed to simultaneously receiving GM and CM as a two-bottle choice (bottles placed next to each other; random order) for circa 1 h per day on two days in their homecage. Then, on the experimental day, chow and water were removed from cages and GM and CM (at room temperature) were given to the animals for 2 h (11:00–13:00). Spillage (g) from each individual bottle

*<sup>2.5.</sup> Energy Deprivation-Induced Intake of Individually Presented GM- and CM-Enriched Chow in Sated Adult Mice and Rats*

was recorded before placement into cage. Intakes were measured using a digital scale after 2 h and expressed in grams per gram of body weight. This feeding experiment was conducted in a separate cohort of adolescent and adult animals.
