**Alina R. Izatulina 1,\*, Anton M. Nikolaev 1,2, Mariya A. Kuz'mina 1, Olga V. Frank-Kamenetskaya <sup>1</sup> and Vladimir V. Malyshev <sup>3</sup>**


Received: 24 April 2019; Accepted: 16 May 2019; Published: 18 May 2019

**Abstract:** The effect of bacteria that present in the human urine (*Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae,* and *Staphylococcus aureus*) was studied under the conditions of biomimetic synthesis. It was shown that the addition of bacteria significantly affects both the phase composition of the synthesized material and the position of crystallization boundaries of the resulting phosphate phases, which can shift toward more acidic (struvite, apatite) or toward more alkaline (brushite) conditions. Under conditions of oxalate mineralization, bacteria accelerate the nucleation of calcium oxalates by almost two times and also increase the amount of oxalate precipitates along with phosphates and stabilize the calcium oxalate dihydrate (weddellite). The multidirectional changes in the pH values of the solutions, which are the result of the interaction of all system components and the crystallization process, were analyzed. The obtained results are the scientific basis for understanding the mechanisms of bacterial involvement in stone formation within the human body and the creation of biotechnological methods that inhibit this process.

**Keywords:** pathogen crystallization; biomimetic synthesis; renal stone; calcium oxalate; apatite; brushite; struvite; octocalcium phosphate; whitlockite; *Escherichia coli*; *Klebsiella pneumoniae*; *Pseudomonas aeruginosa*; *Staphylococcus aureus*
