*2.2. Biochemical Analysis*

The quantification of sugars and acids throughout the fermentation assays were done by HPLC (Waters Corporation, Milford, MA, USA), equipped with a 515 HPLC Pump (Waters Corporation, Milford, MA, USA) and incorporated with a Refractive Index Detector (RID) 486 (Waters Corporation, Milford, MA, USA). Prior to injection, samples were centrifuged at 10.000 rpm/10 min and the supernatants were filtered through a Millipore membrane with a pore size of 0.2 μm. Samples were injected in a Schodex SC-1011 column (Waters Corporation, Milford, MA, USA) and separations were achieved at 50 ◦C, using 5 mM sulphuric acid as mobile phase (isocratic elution), at a flow rate of 0.6 mL.min<sup>−</sup>1. Calibration curves and peak integration were performed as already described by Santos et al. [41].
