2.4.5. Microbial Analysis

Enumeration of Aerobic Microorganisms at 30 ◦C (AM), Psychrotrophic Microorganisms (PM) and Lactic Acid Bacteria (LAB) were performed in duplicate plates as previously described by Santos et al. [41]. Counts were performed for lettuce shreds before and after sanitizing treatments, to monitor microbial development during storage at 1, 3, 5, 7, 9 and 10 days in three independent trials.
