*2.5. DNA Extraction, Amplification and Sequences*

Genomic DNA was extracted from entire individuals of *Paraturbanella tricaudata* species nova (sp. nov.) using a QIAmp DNA Micro Kit (Quiagen, Hilden, Germany), following the manufacturer's instructions. PCR amplification was performed in a 20 μL reaction mixture containing 3 μL of genomic DNA, 13.5 μL of water, 2 μL of 10x buffer, 0.5 μL of dNTP, 0.2 μL of Taq Platinum (Quiagen) and 0.4 μL (4 pmol) of specific primers [4]. The DNA fragments were sequenced using BigDye Terminator reactions in a 3500xL Genetic Analyzer (Life Technologies, Carlsbad, CA, USA) at the CBMEG laboratory (Campinas, Brazil). The 18S rDNA and 28S rDNA sequences of *Paraturbanella tricaudata* sp. nov. were deposited in GenBank (Table 1).
