**Agata Borowik, Jadwiga Wyszkowska \* and Jan Kucharski**

Department of Microbiology, University of Warmia and Mazury in Olsztyn, 10-727 Olsztyn, Poland; agata.borowik@uwm.edu.pl (A.B.); jan.kucharski@uwm.edu.pl (J.K.)

**\*** Correspondence: jadwiga.wyszkowska@uwm.edu.pl

Received: 2 April 2020; Accepted: 22 May 2020; Published: 26 May 2020

**Abstract:** Today, various grass species are important not only in animal feeding but, increasingly often, also in energetics and, due to esthetic and cultural values, in landscape architecture. Therefore, it is essential to establish the roles various grass species and their functional forms play in modifying soil bacteriobiome and enzymatic activity. To this end, a pot experiment was conducted to examine effects of various fodder grass and lawn grass species on the bacteriobiome and biochemical properties of soil. Nonsown soil served as the control for data interpretation. Analyses were carried out with standard and metagenomic methods. The intensity of effects elicited by grasses depended on both their species and functional form. More favorable living conditions promoting the development of soil bacteria and, thereby, enzymatic activity were offered by fodder than by lawn grass species. Among the fodder grasses, the greatest bacteriobiome diversity was caused by sowing the soil with *Phleum pratense* (Pp), whereas among lawn grasses in the soil sown with *Poa pratensis* (Pr). Among the fodder grasses, the highest enzymatic activity was determined in the soil sown with *Lolium* x *hybridum* Hausskn (Lh), and among the lawn grasses—in the soil sown with *Lolium perenne*. Sowing the soil with grasses caused the succession of a population of bacterial communities from r strategy to k strategy.

**Keywords:** fodder grasses; lawn grasses; soil bacteria; soil enzymes
