*2.5. Determination of Protein Concentrations*

Protein concentrations were determined as described by Bradford et al. using bovine serum albumin (BSA) as a standard [20]. Protein samples were diluted to fit the absorption range of the standard concentrations to a final volume of 100 μL and mixed with 900 μL of Bradford reagent (Sigma, B6916). After vigorous mixing and incubating at room temperature for 10 minutes, absorbance was measured at 595 nm using UV-visible spectrophotometer (Biochrom–Libra S22, Cambridge, UK).
