*2.4. Surface Hydrophobicity*

Surface hydrophobicity was measured as described by Chelh et al. [20]. The abovementioned extracted myofibrillar protein was adjusted to 1 mg/mL with phosphate buffer solution (pH 7.0). Two hundred milliliters of bromophenol blue (1 mg/mL) were mixed with 1 mL of protein solution, followed by constant oscillation for 15 min to react sufficiently. As for the control group, the same procedure was implemented using phosphate solution to replace the extracted myofibrillar protein. Then, centrifugation was done at 4 ◦C and 2000× *g* for 15 min. The supernatant was diluted 10 times, the absorbance of which was measured at 595 nm. Surface hydrophobicity was expressed by the following formula: bromophenol blue/mg protein = 200 μg × (OD control − OD sample)/OD control.
