2.4.4. Real-Time PCR Reaction Conditions

Amplification was performed with LightCycler 480II (Roche, Basel, Switzerland) using LightCycler 480 SYBR Green I Master (Roche, Basel, Switzerland) in a volume of 10 mL per sample (5.5 μL premix + 4.5 μL DNA) in 45 cycles according to thermal profiles specific to each *Fusarium* species. The primers used for each researched *Fusarium* species were shown in Table 2.


**Table 2.** Sequences and names of *Fusarium* species specific primers
