*4.7. Other Fusarium Metabolites*

No T-2 toxin or HT-2 toxins were reported in the current investigated wheat samples compared to the notable percentages of European contaminated wheat samples [8]. Equisetin occurred sporadically in some samples due to natural infection by some other *Fusarium* species that are capable of producing it [67]. Besides *Aspergillus*, the main producers of antibiotic Y are *F. avenaceum* and *F. acuminatum*, but as *F. avenaceum* occurred in lower abundance, it was expected that antibiotic Y occurred only in one sample [68].

### *4.8. Alternaria, Aspergillus Mycotoxins and Other Metabolites*

In the current research, besides *Fusarium* mycotoxins/metabolites, *Alternaria* and *Aspergillus* mycotoxins and some other metabolites were detected. This is in accordance with previous research where in field experiments, mycotoxins produced by several di fferent *Fusarium* spp. were detected together with traces of those produced by *Alternaria* spp. [69]. Commonly, mycotoxinogenic fungi are divided into two groups: preharvest (mainly *Fusarium* species) and postharvest (mainly *Aspergillus* and *Penicillium* species) fungi.

The results for most *Alternaria* mycotoxins are in accordance with research where contamination levels in grains were <100 μg/kg and maximum concentrations were <1000 μg/kg [70]. However, the maximum observed tenuazonic acid (TeA) contamination level in wheat was 4224 μg/kg, which is a much higher concentration than that in the current research, i.e., 125 μg/kg in inoculated samples and 138 μg/kg in naturally-infected samples. The higher presence of *Alternaria* mycotoxins in naturally infected samples is expected, since the *Alternaria* spp. did not have to compete with high levels of used *Fusarium* species. Therefore, higher levels of *Alternaria* mycotoxins were documented. It is worth noting that there were statistically significantly higher levels of tentoxin and infectopyron in naturally-infected samples compared to inoculated samples (*p* = 0.03).

Kojic acid, which is produced by *Aspergillus* and *Penicillium* species [71], was observed in two inoculated samples in the current research.
