*3.3. Mycotoxin Production Dynamics*

Mycotoxin production was significantly impacted by the aw treatment (*p*-value < 0.0001 and *p*-value = 0.0331 for DON and ZEN, respectively) with higher production occurring at 0.97 aw. DON production could be quantified from day 4 for the wetter 0.97 aw (Figure 5b) condition and from day 6 in the drier 0.95 aw treatment (Figure 5a). For ZEN production, this was predominantly found from day 6 onwards in both aw treatments (Figure 5). ZEN increased markedly by the end of the experimental period (10 days). Unlike for colonisation of the wheat grain, the inoculum position did not significantly impact either DON or ZEN production.

**Figure 5.** Temporal deoxynivalenol (DON) and zearalenone (ZEN) mycotoxin production from each inoculation position at the two water activity treatments examined. (**a**) DON at 0.95 aw; (**b**) DON at 0.97 aw; (**c**) ZEN at 0.95 aw; and (**d**) ZEN at 0.97 aw. Bars show the standard deviation of the mean.
