**5. Conclusions**

This study detected the presence of a broad range of metabolites in wheat, and future attention should be paid not only to the mycotoxins addressed by regulations, but also to emerging and modified mycotoxins/metabolites. The results showed:


More wheat varieties included in the research could prove to be a better step towards identifying the mycotoxins in wheat in Croatia, which will be examined in our next investigation.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-2607/8/4/578/s1, Figure S1: The boxplot diagrams for the statistically significant di fferences between two treatments (inoculated and naturally-infected) for the major *Fusarium graminearum* and *F. culmorum* metabolites. Figure S2: The boxplot diagrams for the statistically significant di fferences between two treatments (inoculated and naturally-infected) for the other detected metabolites.

**Author Contributions:** Conceptualization, investigation, writing—original draft preparation, project administration V.S.; writing—review and editing, software Z.K.; writing—review and editing, investigation K.P.; resources G.V. and G.D.; writing—review and editing, methodology, M.S. and R.K.; writing—review and editing, formal analysis, B.Š.; All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the European Union, who provided the EUROPEAN REGIONAL DEVELOPMENT FUND, gran<sup>t</sup> number KK.01.1.1.04.0067, and the bilateral corporation Hungary–Croatia.

**Conflicts of Interest:** The authors declare no conflict of interest.
