*3.6. Correlation Between Experimental Components*

Result's correlations of the evaluation of conventional wheat material showed that the proportion of the dead seed was poorly correlated with the germination energy, but highly negatively correlated with germination capacity (Table 7). In the case of organic seed, the dependence was the same, except that the negative values of the correlation coe fficients were higher (Table 8). The proportion of abnormal seedlings was significantly negatively correlated with the energy and germination capacity, as well as the number of dead seeds in conventional material. In contrast to the results of organic material, where the relevant dependencies for these traits were not found. However, organic material has demonstrated a highly significant negative relationship between the proportion of fresh ungerminated seeds and the energy and germination capacity and a positive correlation between fresh ungerminated and dead seed.

In conventional and ecological material, the same negative relationship between FHBi and germination was found, and the positive relationship between FHBi and the share of dead seeds occurred. These two parameters in conventional samples correlated also significantly with concentration of type B trichothecenes in grain.

We observed significant e ffect of colonization of kernels with *Fusarium* species on seed quality (Tables 7 and 8). The proportion of dead seed in conventional material was highly correlated with the quantity of the DNA of *F. graminearum*, while in organic material with the amount of DNA of *F. poae*, *F. sporotrichioides,* and total *Fusarium* DNA.

There was lack of correlation between Fusarium head blight index and amount of ERG and type A and B trichothecenes in grain in both variants (Tables 7 and 8). However, in conventional samples positive tendency FHBi versus type B trichothecenes was observed and the same was found for FHBi versus type A trichothecenes in organic samples. Fusarium head blight index correlated significantly with concentration of DNA of three *Fusarium* species—*F. avenaceum*, *F. graminearum,* and *F. poae* in both variants. In organic variant FHBi correlated significantly also with *F. sporotrichioides* DNA concentration. No correlation was found with *F. culmorum* DNA. Ergosterol content in grain did not correlated with type A or B trichothecenes as well as with DNA concentration of *Fusarium* species.

In samples from conventional field amount of type B trichothecenes correlated highly significantly with *F. graminearum* DNA but not with *F. culmorum* DNA. Contradictory, in organic samples *F. graminearum* did not correlate with type B trichothecenes and for *F. culmorum* there was found positive tendency however not statistically significant. Regarding specific toxins in conventional samples *F. graminearum* correlated significantly with DON amount (*r* = 0.531) and for *F. culmorum* some positive tendency was observed for FUS-X and 15-AcDON. In organic samples, only correlation of *F. culmorum* with 3-AcDON (*r* = 0.421) was found. There was no significant correlation between amount of type A trichothecenes and potentially producing species *F. sporotrichioides* and *F. poae*.

Amounts of DNA of three *Fusarium* species (*F. avenaceum*, *F. graminearum* and *F. poae*) in grain form both variants correlated statistically significantly (Tables 7 and 8). *Fusarium sporotrichioides* DNA concentration correlated with *F. avenaceum* and *F. poae DNA*. *Fusarium culmorum* DNA concentration did not correlated with the other species.


*Microorganisms* **2019**, *7*, 439


*Microorganisms* **2019**, *7*, 439
