2.4.1. Preparation of the Colloidal Suspension of CNCs for Cell-Based Assays

For cytotoxicity assays, the CNC suspensions were prepared at 1 mg/mL in ultrapure water. The CNC suspensions were vortexed for 15 s, sonicated for 2 min at 70 output, followed by filtration with 0.45 μm polytetrafluoroethylene (PTFE) filter and autoclaved at 121 ◦C, 15 psi for 30 min. The suspensions were aliquoted and kept at −20 ◦C for future use.
