*2.1. Animal Experiments*

C57BL/6 male mice were obtained from Daehan Biolink (Dae-Jeon, South Korea) and housed at four per cage in a temperature controlled room under a 12 h light/12 h dark cycle. In the period mice (six weeks) were fed a normal diet containing 4.0% (*w*/*w*) total fat (Rodent NIH-31 Open Formula Auto; Zeigler Bros., Inc., Gardners, PA, USA). Gastrocnemius muscle tissues were collected after 10 or 20 weeks. After collection, muscle tissues were fixed and stored at −80 ◦C until required for RNA and protein extraction or fixed overnight at 4 ◦C for paraffin-embedded tissue blocks to be used in immunohistochemistry. All experimental were done by following the guidelines issued by the Institutional Animal Care and Use Committees of the Catholic University of Daegu (IACUC-2017-051).
