*2.6. Quantitative Real Time PCR*

We added 7.5 μL Fast SYBR Green master mix (Fischer Scientific, 10556555, Pittsburgh, PA, USA), 2.5 μL primer mix and 5 μL cDNA in duplo in a 48-well plate. The program ran on the StepOne real time PCR (Applied Biosystems, Foster City, CA, USA) was 20 s at 95 ◦C holding stage, 40 times 3 s 95 ◦C step 1 and 30 s 60 ◦C step 2 cycle stage, 15 s 95 ◦C, 1 min 60 ◦C and 15 s 95 ◦C. *Gapdh* was used as a housekeeping gene to correct for cDNA input. The efficiency of all used primers (Table 1) was tested.


**Table 1.** Primers for qPCR.
