*2.9. Statistical Analysis*

Graphs were made in Prism version 8 (GraphPad software, San Diego, CA, USA). All data were presented as mean + standard error of the mean (SEM). The data were normalised by the values of a control group or of control cells at 0 h. In graphs of time-dependent relative mRNA expression, values of control cells at 0 h were not presented. Statistical analysis was performed in SPSS version 25 (IBM, Amsterdam, The Netherlands). Significance in the difference between two groups was determined by independent t-test. Statistical significance for multiple comparisons was determined by one-way analysis of variance (ANOVA) or two-way ANOVA with post-hoc Bonferroni corrections. Significance was set at \* *p* < 0.05.
