*2.10. RT-PCR*

Exosomes RNA was synthesized into cDNA and 2 μL cDNA and gene-specific primers (10 pmole, 2 μL) were used for PCR, which was performed using a 2720 Thermal Cycler PCR machine with PCR Master mix (Genetbio, Daejeon, Korea). The PCR conditions were as follow; denaturation 95 ◦C for 30 s, annealing at 59 ◦C for 30 s, extension at 72 ◦C, post-extension 72 ◦C for 5 min followed by holding (40 cycles). The PCR product was examined by performing electrophoresis on agarose gel.
