*2.7. Lentiviral Transduction*

BMDM from WT mice were transduced with a lentivirus carrying a scrambled sequence or a shNfix [27]. Transduction was performed in suspension (in DMEM 20% FBS), at a MOI of 10 and in the presence of Polybrene (8 μg/mL, Sigma-Aldrich). After O/N incubation, the medium was changed and cells were treated with puromycin (2 μg/mL, Sigma-Aldrich).
