**Laura Forcina, Marianna Cosentino and Antonio Musarò \***

Laboratory affiliated to Istituto Pasteur Italia—Fondazione Cenci Bolognetti, DAHFMO-Unit of Histology and Medical Embryology, Sapienza University of Rome, Via Antonio Scarpa, 14, 00161 Rome, Italy; laura.forcina@uniroma1.it (L.F.); marianna.cosentino@uniroma1.it (M.C.)

**\*** Correspondence: antonio.musaro@uniroma1.it; Tel.: +39-06-4976-69-56; Fax: +39-06-4462-854

Received: 26 April 2020; Accepted: 20 May 2020; Published: 22 May 2020

**Abstract:** Despite a massive body of knowledge which has been produced related to the mechanisms guiding muscle regeneration, great interest still moves the scientific community toward the study of different aspects of skeletal muscle homeostasis, plasticity, and regeneration. Indeed, the lack of effective therapies for several physiopathologic conditions suggests that a comprehensive knowledge of the different aspects of cellular behavior and molecular pathways, regulating each regenerative stage, has to be still devised. Hence, it is important to perform even more focused studies, taking the advantage of robust markers, reliable techniques, and reproducible protocols. Here, we provide an overview about the general aspects of muscle regeneration and discuss the different approaches to study the interrelated and time-dependent phases of muscle healing.

**Keywords:** muscle regeneration; inflammatory response; satellite cells; cell precursors; experimental methods; stem cell markers; muscle homeostasis
