*3.9. GC-MS Analysis*

The analyses were performed on a GC-MS system consisting of high-resolution SYNAPT G2 (Waters, Milford, MA, USA) instrument equipped with a quadrupole-time-of-flight (QTOF) analyzer using atmospheric pressure ionization (API) in positive ionization mode. Compounds were separated on a DB-5 capillary column Agilent J&W DB-5ms column (250 μm × 30 m, 0.25 μm film) (Agilent, Santa Clara, CA, USA). The GC parameters were as follows: the column temperature was maintained at 90 ◦C for 1 min, then raised to 200 ◦C (20 ◦C/min) and to 300 ◦C (5 ◦C/min), and held at 300 ◦C for 2 min. Fatty acids were identified by comparison of retention time, molecular formula obtained by their high-resolution molecular ion [M + H]+, and mass spectral data with FAME standards (Supelco-37) and a NIST library.
