*3.11. Statistical Analysis*

Statistical analyses were performed by Graphpad Prism 6.0. An unpaired Student's *t*-test was used to compare two groups; analysis of variance (ANOVA) and Dunnett's *post hoc*-test was applied to compare control with all other treatments, instead a Bonferroni post-test was applied to analyze data in oxidative stress experiments. Differences were considered statistically significant for values *p* < 0.05. All assays were replicated different time (*n* = 6) and data are represented as mean ± standard deviation (SD). The half maximal inhibitory concentration (IC50) for fractions was calculated using the same program Graphpad Prism 6.0.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1660-3397/17/2/134/s1, Video S1: The sampling site of Rhizostoma pulmo jellyfish at Marina di Ginosa, Ionian Sea, Italy (@Antonella Leone CNR-ISPA).

**Author Contributions:** Conceptualization, data analysis, methodology, writing and review manuscript, funding acquisition, project administration, A.L.; performing experiments, data analysis, writing initial draft preparation, S.D.D.; performing experiments, data analysis, G.D.R.; set up first experiments, M.P.; manuscript review, S.P.

**Funding:** This work was supported by the project GoJelly—A gelatinous solution to plastic pollution—under gran<sup>t</sup> agreemen<sup>t</sup> no. 774499 European Union's Horizon 2020 programme H2020-EU.3.2.5, BG-07-2017—Blue green innovation for clean coasts and seas.

**Acknowledgments:** The Authors thanks Leone D'Amico for his valuable technical support and Fabio Minerva, Associazione Master Wave, for the high professional support during jellyfish sampling.

**Conflicts of Interest:** The authors declare no conflict of interest.
