4.6.1. Sample Preparation

Jellyfish mucus and tissue homogenate were precipitated with three times the volume of methanol. The precipitates were removed by 4000 rpm centrifugation for 10 min at 4 ◦C after shaking of the mixture for 1 min. The clear supernatant (100 μL) was transferred to a sampling vial for UPLC-QTOF-MS/MS analysis. Meanwhile, the 100 μL aliquot of each sample was also transferred to a sampling vial and mixed as a quality control (QC) to check the stability of the system and method.
