*3.8. Genome Assembly*

The quality control of reads was checked with FASTQC software [43] to evaluate the GC%, number of k-mers, sequence length, and total reads. Trimmomatic v0.36 [44] was used to filter low quality sequences and adapters. Filtered reads were assembled with SPAdes v3.11.0 [45] using default k-mer lengths. The obtained contigs were evaluated with QUAST tool [46] to select the best quality contig. Finally, Prokka [47] was used to annotate the draft genome.

### *3.9. Secondary Metabolites Gene Clusters Search*

The online platform of Antismash [30] was used to detect the secondary metabolites gene clusters present in the draft genome.

### *3.10. Antibiotic Activity Test*

To test the antibiotic activity, we used the disc diffusion method [48] as a primary indicator. Thus, compound (**1**) and (**2**) were tested to determine their activity on *Staphylococcus lentus* DSM 20352T, and *Escherichia coli* DSM 498T. These bacteria were cultured in GYM medium (4 g glucose, 4 g yeas<sup>t</sup> extract, 10 g malt extract, 2 g CaCO3, 1 L deionized water, pH = 7.2, and 12 g agar). Lupinacidin A (**1**), and Galvaquinone B (**2**) were transferred to a paper disc to reach a final concentration of 25 μg and 50 μg each in triplicate. Additionally, we used an antibiotic susceptibility disc of streptomycin (Oxoid®, Columbia, MD, USA) as a positive indicator of antibiotic activity. The plates were inoculated with fresh culture of *Staphylococcus lentus* DSM 20352T, and *Escherichia coli* DSM 498T, and incubated at 37 ◦C for 24 h. After the incubation period, the inhibition zone was measured and registered.
