*3.5. Isoelectric Focussing*

Analytical isoelectric focusing polyacrylamide gel electrophoresis was performed on a HPETM BlueHorizonTM system (Serva Electrophoresis GmbH, Heidelberg, Germany) using ServalytTM PrecotesTM Precast Gels (Serva Electrophoresis GmbH) with an immobilized pH gradient of pH 3 to 10. To determine the isoelectric points of the enzymes, marker proteins (IEF-Marker 3-10, Serva Electrophoresis GmbH) were used. Gels were stained with Coomassie Brilliant Blue, or for specific visualization of peroxidases, with 1% (*w*/*v*; 9.2 mM) phenylendiamine and 1% (*w*/*v*; 10.6 mM) urea peroxide (dissolved in 100 mM sodium acetate buffer pH 3.5) at RT until a yellow band was observed.
