*Article* **Mapping the Biotransformation of Coumarins through Filamentous Fungi**

**Jainara Santos do Nascimento 1, Wilson Elias Rozo Núñez 1, Valmore Henrique Pereira dos Santos 1, Josefina Aleu 2, Sílvio Cunha <sup>1</sup> and Eliane de Oliveira Silva 1,\***


Received: 11 September 2019; Accepted: 27 September 2019; Published: 29 September 2019

**Abstract:** Natural coumarins are present in remarkable amounts as secondary metabolites in edible and medicinal plants, where they display interesting bioactivities. Considering the wide enzymatic arsenal of filamentous fungi, studies on the biotransformation of coumarins using these microorganisms have great importance in green chemical derivatization. Several reports on the biotransformation of coumarins using fungi have highlighted the achievement of chemical analogs with high selectivity by using mild and ecofriendly conditions. Prompted by the enormous pharmacological, alimentary, and chemical interest in coumarin-like compounds, this study evaluated the biotransformation of nine coumarin scaffolds using *Cunninghamella elegans* ATCC 10028b and *Aspergillus brasiliensis* ATCC 16404. The chemical reactions which were catalyzed by the microorganisms were highly selective. Among the nine studied coumarins, only two of them were biotransformed. One of the coumarins, 7-hydroxy-2,3-dihydrocyclopenta[*c*]chromen-4(1*H*)-one, was biotransformed into the new 7,9-dihydroxy-2,3-dihydrocyclopenta[*c*]chromen-4(1*H*)-one, which was generated by selective hydroxylation in an unactivated carbon. Our results highlight some chemical features of coumarin cores that are important to biotransformation using filamentous fungi.

**Keywords:** coumarin; biotransformation; filamentous fungi; selective hydroxylation
