*3.4. Gel Electrophoresis*

SDS-PAGE analysis was performed as described elsewhere [43]. Semi-native PAGE was performed under non-denaturing conditions using 12% gels. For this, samples were prepared with a native loading buffer (without DTT and without 2% (*w*/*v*; 6.9 mM) SDS) and gel electrophoresis was performed at 10 mA per gel and 4 ◦C. Gels were stained with 0.5 mM ABTS (dissolved in 100 mM sodium acetate buffer pH 3.5 or 4.5) in the presence of 100 μM hydrogen peroxide for detection of peroxidases. For deglycosylation, samples were treated with 1 μL (500 U) endoglycosidase H (EndoH, New England BioLabs, Ipswich, MA, USA) in 20 μL for 2 h at 37 ◦C before gel electrophoresis.
