*4.1. Microorganisms, Animal Cells, and Chemicals*

*Deinococcus geothermalis* DSM11300 (BCRC17378) and mouse macrophage cells RAW 264.7 (BCRC60001) were obtained from the Bioresources Collection and Research Center (BCRC, Food Industry Research and Development Institute, Hsinchu, Taiwan), and cultivated according to the BCRC protocol. *E. coli* BL21 (DE3) and the *pET-Duet-1* expression plasmid were obtained from the Novagen Company (Madison, WI, USA). Restriction enzymes and DNA modified enzymes were obtained from New England Biolabs (Ipswich, MA, USA). 8-OHDe was prepared according to Dr. Wu's method [18]. IPTG, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), LPS, and Greiss reagent were purchased from Sigma (St. Louis, MO, USA). The other reagents and solvents used are commercially available.
