4.8.3. Evaluation of [68Ga]**3** in Mice Bearing LS174T Tumor Xenografts

Four female nude BALB/c mice bearing subcutaneous LS174T tumor xenografts were administered i.v. with **5** (100 μg) in PBS (50 μL); four mice without pre-treatment were included as controls (mean size of 126.2 <sup>±</sup> 26.0 mm3). After 24 h [68Ga]**<sup>3</sup>** (mean 6 MBq, 10–13 <sup>μ</sup>g, 8–10 nmol) in <sup>&</sup>lt; 10% EtOH/PBS (100μL) was administered i.v. PET/CT images were obtained 2 h p.i. Following last scan, the mice were euthanized and the tumor, blood, and muscle tissue were collected and weighed. Sample radioactivity was measured using a gamma counter and the %ID/g values were calculated after correcting for decay and injected amount of radioactivity.
