*Article* **Monitoring the Bioactive Compounds Status in** *Olea europaea* **According to Collecting Period and Drying Conditions**

**Amani Taamalli 1, Jesus Lozano Sánchez 2,3,\*, Haifa Jebabli 1, Najla Trabelsi 1, Leila Abaza 1, Antonio Segura Carretero 3,4, Jae Youl Cho 5 and David Arráez Román 3,4**


Received: 4 January 2019; Accepted: 6 March 2019; Published: 12 March 2019

**Abstract:** Polyphenols and triterpenoids in olive have relevant importance both in the physiology of the plant and the nutritional and biological value of its products. Olive leaf extracts are of special interest for their numerous health-promoting properties. The present research is investigating the occurrence of phytochemicals in supercritical fluid extracts from leaves with regard to collection time and drying temperature. The phytochemical profiles of the olive leaf extracts were determined by reversed-phase high-performance liquid chromatography (HPLC) coupled to electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) detection. The main extracted phytochemicals were phenols and terpenoids. A significant variation in the amounts of the different components was observed as a function of the different drying temperature and collecting time (*p* < 0.05). Among samples, the maximal contents of polyphenols and secoiridoid derivatives were found in the extracts from olive leaves collected in November and dried at 120 ◦C, whereas triterpenoids showed the highest content in fresh leaves collected in August.

**Keywords:** *Olea europaea*; supercritical fluid extraction; triterpenoids; polyphenols; olive leaves
