**4. Patents**

(WO2018224939) Gene cluster for the biosynthesis of albucidin.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-2607/8/2/237/s1, Figure S1: 1H NMR spectrum of albucidin (500 MHz), Figure S2: The genes *albA* and *albB* with the mapped point mutations identified in the course of NTG-mutagenesis, Figure S3: High resolution HPLC-MS analysis of albucidin production by *Streptomyces albus* 1K1, Figure S4: HPLC-MS analysis of albucidin production by *Streptomyces albus* strain harboring 1K1 BAC and its derivatives with gene deletions, Figure S5: HPLC-MS analysis of albucidin production by *Streptomyces albus* 1K1 BAC (**a**) and *Streptomyces albus* 2D4 BAC (**b**), Figure S6: HPLC-MS analysis of albucidin production by *Streptomyces albus* strain harboring 1K1 BAC and its derivatives with gene deletions, Figure S7: HPLC-MS analysis of albucidin production by *Streptomyces albus* strain harboring full length 1K1 BAC (**a**) and minimized 1K1\_alb\_act BAC, containing only transcriptionally activated *albA–C* operon (**b**), Table S1: Bacterial strains used in the study, Table S2: Plasmids and BACs used in the study, Table S3: Primers used in this study. References [26,27] are cite d in the supplementary materials.

**Author Contributions:** L.P., T.S., M.M., and A.L. designed the experiments; M.M. and B.R. performed the experiments; M.S. performed structure elucidation studies; M.M., B.R., and A.L. analysed the data and wrote the manuscript, and all authors reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was partially supported by the BMBF grant "MyBio" 031B0344B.

**Conflicts of Interest:** The authors declare no conflict of interest.
