*2.6. Strains Culture, Maintenance, and Secondary Metabolites Production*

The same culture media and protocols were employed for both isolates *Streptomyces* sp. SM17 and *Streptomyces albidoflavus* J1074. Glycerol stocks were prepared from spores collected from soya-mannitol (SM) medium after 8 days of cultivation at 28 ◦C and preserved at −20 ◦C. To verify the secondary metabolites production profile, spores were cultivated for 7 days on SM agar medium at 28 ◦C, then pre-inoculated in 5 mL TSB medium, and cultivated at 28 ◦C and 220 rpm for 2 days. Then, 10% (*v*/*v*) of the pre-inoculum was transferred to 30 mL of the following media: TSB; SYP-NaCl (1% starch, 0.4% yeast extract, 0.2% peptone, and 0.1% NaCl); YD (0.4% yeast extract, 1% malt extract, and 4% dextrin pH 7.0); P1 (2% glucose, 1% soluble starch, 0.1% meat extract, 0.4% yeast extract, 2.5% soy flour, 0.2% NaCl, and 0.005% K2HPO4 pH 7.3); P2 (1% glucose, 0.6% glycerol, 0.1% yeast extract, 0.2% malt extract, 0.6% MgCl2.6H2O, 0.03% CaCO3, and 10% sea water); P3 (2.5% soy flour, 0.75% starch, 2.25% glucose, 0.35% yeast extract, 0.05% ZnSO4 × 7H2O, and 0.6% CaCO3 pH 6.0); CH-F2 (2% soy flour, 0.5% yeast extract, 0.2% CaCO3, 0.05% citric acid, 5% glucose, and pH 7.0); SY (2.5% soluble starch, 1.5% soy flour, 0.2% yeast extract, and 0.4% CaCO3 pH 7.0); Sporulation medium (2% soluble starch and 0.4 yeast extract); and Oatmeal medium (2% oatmeal). These were cultivated at 28 ◦C and 220 rpm for 4 days in TSB; and for 8 days in SYP-NaCl, YD, SY, P1, P2, P3, CH-F2, Sporulation, and Oatmeal media. Once the bioprocess was completed, the broth was frozen at −20 ◦C for further chemical analysis.
