*2.3. Prediction of Secondary Metabolites Biosynthetic Gene Clusters*

In order to assess the similarities and differences between the *Streptomyces* isolates belonging to the *albidoflavus* phylogroup, in regard to their potential to produce secondary metabolites, BGCs were predicted in their genomes, using the antiSMASH (version 5 available at https://docs.antismash. secondarymetabolites.org/) program [42]. The predicted BGCs were then processed using the BiG-SCAPE program (version 20190604, available at https://git.wageningenur.nl/medema-group/BiG-SCAPE) [43], with the MiBIG database (version 1.4 available at https://mibig.secondarymetabolites.org/) as reference [44], and similarity clustering of gene cluster families (GCFs) was performed. The similarity network was processed using Cytoscape (version 3.7.1, available at https://cytoscape.org/) [45].
