**4. Conclusions**

A novel strain, NEAU-H2T, with antifungal activity was isolated from the rhizosphere soil of wheat (*Triticum aestivum L.*). Four compounds, including one new compound, along with three known congeners (3-Acetylamino-N-2-thienyl-propanamide, β-adenosine, 2-minaline), were isolated. Morphological and chemotaxonomic features together with phylogenetic analysis and genomes suggested that strain NEAU-H2<sup>T</sup> belonged to the genus *Streptomyces*. Cultural and biochemical characteristics combined with DNA–DNA relatedness values clearly revealed that strain NEAU-H2<sup>T</sup>

was differentiated from its closely related strains. Based on the polyphasic taxonomic analysis, it is suggested that strain NEAU-H2<sup>T</sup> represents a novel species of the genus *Streptomyces*, for which the name *Streptomyces triticiradicis* sp. nov. is proposed. The type strain is NEAU-H2T (=CCTCC AA 2018031<sup>T</sup> = DSM 109825T).

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-2607/8/1/77/s1.

**Author Contributions:** Z.Y., C.H., B.Y. and J.Z. performed the experiments. S.H. and Y.Y. analyzed the data. Z.Y. and C.H. wrote the paper. C.L. and W.X. designed the experiments and reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by the Academic Backbone Project of Northeast Agricultural University (Grant No. 17XG17) and Open Project of Key Laboratory of Agricultural Microbiology of Heilongjiang Provincial (NW 2017003).

**Acknowledgments:** We are grateful to Aharon Oren for helpful advice on the species epithet.

**Conflicts of Interest:** The authors declare that they have no conflicts of interest.
