*2.1. General Experimental Procedures*

All strains, plasmids and BACs used in this work are listed in Tables S1 and S2. *Escherichia coli* strains were cultured in LB medium [11]. *Streptomyces* strains were grown on soya flour mannitol agar (MS agar) [12] and in liquid tryptic soy broth (TSB; Sigma-Aldrich, St. Louis, MO, USA). For albucidin production, liquid SG medium [13] was used. The antibiotics kanamycin, apramycin, hygromycin, ampicillin and nalidixic acid were supplemented when required.
