*2.4. Gene Synteny Analysis*

The genome regions previously determined to share similarities with the *S. albidoflavus* LHW3101 *sur* BGC were manually annotated, for the known main biosynthetic genes (*surABCD*), the penicillin binding protein (PBP)-like peptide cyclase and hydrolase *surE* gene, and the gene with regulatory function *surR* [15–19,21]. This was performed using the UniPro UGENE toolkit (version 1.32.0, available at http://ugene.net/) [46], the GenBank database, and the NCBI BLASTN tool (available at https://blast.ncbi.nlm.nih.gov/Blast.cgi, accessed on June 2019) [35–37]. The gene synteny and reading frame analysis was performed using the UniPro UGENE toolkit [46] and the Artemis genome browser (version 18.0.0, available at https://www.sanger.ac.uk/science/tools/artemis) [47].
