2.5.2. Determination of Minimum Inhibitory Concentration

The minimum inhibitory concentration (MIC) value of the partially purified extract (PPE) was determined by micro dilution method. Bacterial and fungal pathogens were grown in sterile broth and 10 μL of log phase culture was added into 96 well micro titre plates. Partially purified fractions were dissolved in 1% DMSO and serially diluted to give required concentrations (1 mg/mL–3.9 μg/mL). Diluted fractions and sterile broth were added into pre-coated microbial cultures, making up a total of volume of 200 μL. The plate was incubated at 37 ◦C and room temperature (for test bacteria and fungi respectively).
