**5. Conclusions**

Strain producing a new compound with strong antitumor activity, isolated from the rhizosphere soil of wheat (*Triticum aestivum* L.) in HeBei province, China. Morphological and chemotaxonomic features together with phylogenetic analysis suggested that strain NEAU-wh3-1 belonged to the genus *Embleya*. Cultural and biochemical characteristics combined with multilocus sequence analysis clearly revealed that strain NEAU-wh3-1 may represent a novel species of the genus *Embleya.* Moreover, eight compounds, including one new compound with higher antitumor activities against three human cell lines, were isolated from the strain.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2076-2607/8/3/441/s1, Table S1: GenBank accession numbers of the sequences used in MLSA; Table S2: MLSA distance values for selected strains in this study; Figure S1: The polar lipids of strain NEAU-wh3-1; Figure S2: Maximum-likelihood tree based on 16S rRNA gene sequences showing relationship between strain NEAU-wh3-1 and related taxa; Figure S3: Maximum-likelihood tree based on multilocus sequence analysis (MLSA)analysis of the concatenated partial sequences (1979 bp) from five housekeeping genes (*atpD*, *gyrB*, *recA*, *rpoB*, and *trpB*) of strain NEAU-wh3-1 (in bold) with related taxa; Figure S4: 1H NMR (400 MHz) spectrum of compound **1** in CDCl3; Figure S5: 13C NMR (150 MHz) spectrum of compound **1** (in CDCl3); Figure S: 1H-1H COSY spectrum (400 MHz) of compound **1** (in CDCl3); Figure S7: HSQC spectrum (400 MHz) of compound **1** (in CDCl3); Figure S8: HMBC spectrum (400 MHz) of compound **1** (in CDCl3); Figure S9: IR spectrum of compound **1**(in EtOH); Figure S10: UV spectrum of compound **1** (in EtOH); Figure S11: The HRESIMS spectrum of compound **1**; Figure S12: 1H NMR (400 MHz) spectrum of compound **2** in CDCl3; Figure S13: 13C NMR (150 MHz) spectrum of compound **2** in CDCl3; Figure S14: The ESI-MS spectrum of compound **2**; Figure S15: 1H NMR (400 MHz) spectrum of compound **3** in CDCl3; Figure S16: The ESI-MS spectrum of compound **3**; Figure S17: 1H NMR (400 MHz) spectrum of compound **4** in CDCl3; Figure S18: The ESI-MS spectrum of compound **4**; Figure S19: 1H NMR (400 MHz) spectrum of compound **5** in CDCl3; Figure S20: The ESI-MS spectrum of compound **5**; Figure S21: 1H NMR (400 MHz) spectrum of compound **6** in CDCl3; Figure S22: The ESI-MS spectrum of compound **6**; Figure S23: 1H NMR (400 MHz) spectrum of compound **7** in CDCl3; Figure S24: ESI-MS spectrum of compound of compound **7**; Figure S25: 1H NMR (400 MHz) spectrum of compound **8** in CDCl3; Figure S26: 13C NMR (150 MHz) spectrum of compound **8** in CDCl3; Figure S27: The HRESIMS spectrum of compound **8**.

**Author Contributions:** H.W., T.S., and W.S. performed the experiments. X.G. prepared the figures and tables. P.C. and X.X. analyzed the data. Y.S. and J.Z. designed the experiments and reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported in part by grants from the Heilongjiang Postdoctoral Fund (LBH-Z17015) and the Scientific Research Foundation for Settled Postdoctoral of Heilongjiang Province (LBH-Q19082).

**Conflicts of Interest:** The authors declare that there are no conflict of interest.
