*2.4. Fermentation*

Isolate *Streptomyces* sp.S2A was inoculated in ISP-2 broth (Himedia) and kept for incubation at the rotary shaker (140 rpm, 28 ◦C) for 14 days. The culture broth obtained was extracted thrice with ethyl acetate (EA, Fisher Scientific, Madison, WI, USA) and concentrated under the rotary evaporator at 50 ◦C.

### *2.5. Antimicrobial Assays*
