*2.7. Hemolytic Activities*

Hemolytic activity was determined by disc diffusion assay using sheep blood agar (SBA) plates (Himedia, Mumbai, India). A total of 10 μL solution containing varying concentrations of the ADR1 metabolites (1000 to 7.8125 μg/mL) were dispensed on discs placed aseptically on the SBA plates and were incubated for 24 h at 37 ◦C. The type of hemolysis was observed as alpha, beta and gamma [37].
