*2.8. In Vitro Antifungal Activity Test of Compounds*

The fungi were retrieved from the storage tube and cultured for seven days at 28 ◦C on PDA. All fungi were further cultured for one week to get new mycelium for the antifungal assays in PDA at 28 ◦C. The medium was mixed with the pathogenic fungi suspension at about 45 ◦C, ensuring the abundance of the strains was about 108 cfu/mL. Next, the mixture was poured on 9-cm Petri dishes. The tested compounds were dissolved in DMSO at a concentration of 2 mg/mL. Each filter paper (5 mm in diameter) was impregnated with 10 μL of the tested compounds. The inoculated Petri dishes were cultured for 3 to 4 d at 28 ◦C. DMSO served as a blank control. The assay was measured three times. The inhibition diameter was measured by the cross bracketing method [46].
