*3.2. Description of Streptomyces triticiradicis sp. nov.*

*Streptomyces triticiradicis* (tri.ti.ci.ra'di.cis. L. neut. n. *triticum* wheat; L. fem. n. *radix* a root; N.L. gen. n. *triticiradicis* of a wheat root).

This is an aerobic gram-staining-positive actinomycete that forms well-developed, branched substrate hyphae and aerial mycelium that differentiate into spiral spore chains consisting of spiny surfaced spores. It has good growth on ISP 1–4, ISP 7 and Nutrient agar media, moderate growth on Bennett's and Czapek's agar media, and poor growth on ISP 5 and ISP 6 media. A dark grayish olive pigment is produced on ISP 6 medium. Growth is observed at temperatures between 4 and 40 ◦C, with an optimum temperature of 28 ◦C. Growth occurs in the pH range from 5.0 to 10.0 (optimum pH 7.0) with 0% to 9.0% (*w*/*v*) NaCl tolerance (optimum 0%–1%). It is positive for coagulation of milk; decomposition of cellulose; hydrolysis of aesculin, starch, and Tweens (20, 40, and 80); and production of H2S and urease; but negative for liquefaction of gelatin, production of catalase, and reduction of nitrate. l-alanine, l-arginine, l-asparagine, l-aspartic acid, creatine, l-glutamic acid, l-glutamine glycine, l-proline, and l-threonine are utilized as sole nitrogen sources but not l-serine or l-tyrosine. d-Fructose, d-galactose, d-glucose, lactose, d-maltose, d-mannose, d-raffinose, l-rhamnose, and d-sucrose are utilized as sole carbon sources but not l-arabinose, dulcitol, meso-inositol, d-mannitol, d-ribose, d-sorbitol, or d-xylose. The cell wall contains LL-diaminopimelic acid as diagnostic diamino acid and the whole cell hydrolysate contains glucose. The major menaquinones are MK-9(H8), MK-9(H6), and MK-9(H4). The polar lipids profile contains DPG, PE, PI, PIM, and PL. Major fatty acids (>10%) are iso-C16:0, anteiso-C15:0, iso-C15:0, and anteiso-C17:0. The DNA G + C content of the type strain is 71.5 mol%.

The type strain is NEAU-H2T (= CCTCC AA 2018031T = DSM 109825T), isolated from the rhizosphere soil of wheat (*Triticum aestivum* L.) collected from Zhumadian, Henan Province, Central China. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NEAU-H2T is MN512450. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession WBKG00000000. The version described in this paper is version WBKG01000000.1.
