*2.9. Gas Chromatography-Mass Spectrometry (GC-MS)*

The analysis of the volatile constituents in extracts was determined by GC-MS technique (Perkin Elmer Clarus, USA). Perkin Elmer Clarus 680 employed a fused silica column, packed with Elite-5MS and the compounds were separated using helium as a carrier gas at a constant flow of 1 mL/min. The injector temperature was kept at 260 ◦C. Oven temperature was set as follows: 60 ◦C (2 min); followed by 300 ◦C at the rate of 10 ◦C min<sup>−</sup>1. The spectrum thus obtained was compared with the database of the already known spectrum of components stored in GC-MS NIST library. The infrared spectrum of the extract was analyzed by FT-IR spectrophotometer in the range of 400–4000 cm<sup>−</sup>1.
