2.5.2. Minimum Inhibitory Concentration (MIC)

The minimum inhibitory concentration assay was determined by micro broth dilution method as previously reported by Siddharth and Rai [21]. The serially diluted fraction of extracts with sterile Mueller Hinton broth (Himedia, India) was added to pre coated microbial cultures in 96-well micro titer plates to give a final concentration of 1–3.8 μg/mL. The titer plate was incubated for 24 h at 37 ◦C. The lowest concentration of extract which completely inhibited the bacterial growth (no turbidity) was considered as MIC. Each test was done in triplicate.
