**4. Conclusions**

The present study was successful in determining the diversity and bioactive potential of actinobacterial isolates from Western Ghats region of Karnataka. Relatively underexplored forest regions of Western Ghats of Karnataka are found to be promising resources for the discovery of natural bioactive metabolites. The isolates showed significant antimicrobial activity against pathogenic Gram-positive MRSA bacteria, Gram-negative bacteria, and fungi. They were also found to possess antioxidant potential. Our studies encourage the exploration of diverse ecosystems for the isolation of new species for novel and biologically active compounds. Further studies are under progress to purify and characterize the crude extracts that may result in the economic production of bioactive compounds for pharmaceutical applications.

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/2076-2607/8/2/225/s1.

**Author Contributions:** Conceptualization, S.S., R.R.V., W.J. and M.S.; Methodology, S.S. and R.R.V.; Formal analysis, S.S. and R.R.V.; Investigation, S.S., Original Draft Preparation, S.S. and R.R.V.; Writing—Reviewing and Editing, S.S., R.R.V., W.J. and M.S.; Visualization, S.S. and R.R.V.; Supervision, R.R.V. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by India Council of Medical Research, New Delhi (ICMR-SRF/AMR/FELLOWSHIPS/5/2019-ECD-II, dated: 27.06.2019).

**Acknowledgments:** The authors would like to acknowledge the facilities provided by University of Mysore. The authors gratefully acknowledge the financial support grant to Mr. Saket Siddharth, ICMR-SRF from India Council of Medical Research, New Delhi (ICMR-SRF/AMR/FELLOWSHIPS/5/2019-ECD-II, dated: 27.06.2019).

**Conflicts of Interest:** The authors declare that they have no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
