*2.2. Isolation and Manipulation of DNA*

The previously constructed BAC library of *Streptomyces albus* subsp. *chlorinus* NRRL B-24108 was used [14]. DNA manipulation, *E. coli* transformation and *E. coli*/*Streptomyces* intergeneric conjugation were performed according to standard protocols [11,12,15]. BAC DNA was purified with the BACMAX™ DNA purification kit (Lucigen, Middleton, WI, USA). Restriction endonucleases were used according to the manufacturer's recommendations (New England Biolabs, Ipswich, MA, USA). All the strains and plasmids are listed in the Tables S1 and S2, respectively.
