**2. Materials and Methods**

#### *2.1. Isolation of Actinobacterial Strain*

Strain NEAU-H2T was isolated from the rhizosphere soil of wheat (*Triticum aestivum* L.) collected from Zhumadian, Henan Province, Central China (32◦98 N, 114◦02 E). The root sample was air-dried for 24 h at room temperature, and then the surface soil was shaken off gently. After, the sample was shaken at 250 rpm in 100 mL of sterile water with glass beads for 30 min at 20 ◦C and then filtered with a single layer of gauze to obtain the rhizosphere soil suspension. The suspension was serially diluted and spread on cellulose-proline agar (CPA) [12] supplemented with cycloheximide (50 mg·L<sup>−</sup>1) and nalidixic acid (20 mg·L<sup>−</sup>1), and cultured at 28 ◦C for 3 weeks. Strain NEAU-H2T was isolated and purified on the International *Streptomyces* Project (ISP) medium 3 [13], and maintained as glycerol suspensions (20%, *v*/*v*) at −80 ◦C.
