*2.9. RT-qPCR*

Total RNA was extracted from liver frozen specimens with or without treatments using an RNeasy kit (Qiagen, Germany). RT-qPCR was performed in an ABI Prism 7300 sequence detecting system (Applied Biosystems, CA, USA). Primers were as follows: 18s rRNA: Sense, 5 -GTA ACC CGT TGA ACC CCA TT-3 ; Antisense, 5 -CCA TCC AAT CGG TAG TAG CG-3 . Ly6G: Sense, 5 -AGA AGC AAA GTC AAG AGC AAT CTC T-3 ; Antisense, 5 -TGA CAG CAT TAC CAG TGA TCT CAG T-3 . CB1: Sense, 5 -GGC GGT GGC CGA TCT C-3 ; Antisense, 5 -CGG TAA CCC CAC CCA GTT T-3 . CB2: Sense, 5 -AGC GCC CTG GAG AAC ATG-3 ; Antisense, AGC TGC TGA TGA ACA GGT ACG A-3 . MCP-1: Sense, 5 -TCT GGG CCT GCT GTT CAC A-3 ; Antisense, 5 -GGA TCA TCT TGC TGG TGA ATG A-3 . IL-1β: Sense, 5 -GCA ACT GTT CCT GAA CTC AAC T-3 ; Antisense, 5 -ATC TTT TGG GGT CCG TCA ACT-3 . IL-6: Sense, 5 -TAG TCC TTC CTA CCC CAA TTT CC-3 ; Antisense, 5 -TTG GTC CTT AGC CAC TCC TTC-3 . CD86: Sense, 5 -TCC AAG TTT TTG GGC AAT GTC-3 ; Antisense, 5 -CCT ATG AGT GTG CAC TGA GTT AAA CA-3 .
