*4.6. Statistical Analysis*

The results were expressed as mean ± standard deviation. In order to test data normality and variance homogeneity, the Kolmogorov–Smirnov test and Levene's F-test were used, respectively. Data with parametric distribution were analyzed using one-way analysis of variance (ANOVA), and the significant differences between treatments (*p* < 0.05) were determined using Tukey's multiple comparison test. Data with nonparametric distribution were analyzed by using Kruskal–Wallis test, and significant differences were determined using box-and-whisker plot graphs. All statistical analyses were performed using the Statgraphics Plus 4.0 (Rockville, MD, USA) software.
