*2.14. Cell Cycle Analysis*

At 24 h prior to transfection, 3.5 × 10<sup>4</sup> T24 cells per well were seeded in a 12-well plate in 1000 μL growth medium and then medium was replaced by 400 μL fresh medium. A total of 100 μL MSN-160 nm-454-GE11 (500 μg mL−<sup>1</sup> in HEPES buffer) loaded with Ctrl RNA or miR200c was added and incubated at 37 ◦C for 4 h. After a selected incubation time, the medium was replaced with fresh medium and cells were incubated for an additional 72 h. Afterwards, cells were washed with PBS and detached with trypsin/EDTA. Cells were washed with PBS twice and suspended in 100 μL PBS. The cell suspension was added dropwise to 0.9 mL cold 70% ethanol and incubated at 4 ◦C for 2 h. Then, cell pellets were suspended in 1 mL PBS after centrifugation and incubated for 15 min at room temperature for counting. A total of 1 × 10<sup>5</sup> cells of each sample was incubated in 300 μL propidium iodide/TritonX-100 containing RNase solution for 15 min at 37 ◦C. For cell cycle analysis, the cells were analyzed by flow cytometry at an excitation wavelength of 488 nm and detection of emission with a 613/20 bandpass filter. Data were recorded by BD LSRFortessa™ (BD Biosciences, USA) and analyzed by FlowJo® 7.6.5 flow cytometric analysis software. All experiments were performed in triplicate.
