*2.13. Luciferase Gene Silencing*

In all experiments, siRNA and miRNA delivery was performed in 96-well plates in triplicate. 3500 T24/eGFPLuc-200cT cells per well were seeded 24 h prior to transfection in 100 μL growth medium. Before transfection, medium was replaced with 80 μL fresh growth medium. 20 μL MSN, MSN-454-PEG and MSN-454-GE11 for RNA delivery (500 μg mL−<sup>1</sup> MSN in HEPES buffer) were added to each well and incubated for 45 min at 37 ◦C followed by a medium exchange. Cells were then incubated with 100 μL fresh medium for an additional 48 h following transfection. After this time, cells were treated with 100 μL cell lysis buffer (Promega (Mannheim, Germany)). Luciferase activity in cell lysate (35 μL) was measured using a luciferin-LAR (1 M glycylglycine, 100 mM MgCl2, 500 mM EDTA, DTT, ATP, coenzyme A) buffer solution on a luminometer for 10 s (Centro LB 960 plate reader luminometer, Berthold Technologies, Bad Wildbad, Germany).
