*2.8. miRNA Binding Assay*

For the miRNA binding assay, a 2.5% agarose gel was prepared by dissolving agarose in TBE buffer (Trizma base 10.8 g, boric acid 5.5 g, disodium EDTA 0.75 g, and 1 L of water) and by heating to 100 ◦C. GelRed® (1:10,000) was added and the agarose gel was cast in the electrophoresis unit. MSN-454-GE11 samples loaded with Ctrl RNA were mixed with 4 μL loading buffer (6 mL glycerol, 1.2 mL of 0.5 M EDTA, 2.8 mL H2O, 0.02 g bromophenol blue) and placed into the gel sample pockets. Electrophoresis was run at 120 V for 90 min.
