2.7.4. Histopathological Analysis

For the histopathological study, explant samples were fixed in phosphate-bu ffered formaldehyde solution (4%, pH 7.2, 0.05 M), embedded in para ffin, sectioned at 5 μm, and stained with hematoxylin and eosin (H&E) and Gomori's trichrome kit (Leica Biosystems, 38016SS1, Nussloch, Germany).

Microscopic sections were analyzed with an Olympus BX43 microscope equipped with a digital camera Olympus XC30 and CellSense software and graded for the amount of tissue reaction.

Sections were scored on the degree of inflammatory infiltrate (including acute and chronic inflammatory cells), fibroblasts and neovascularization. Each histometric parameter was graded on a scale of 0–4 for the amount of tissue reaction: − (not present); sp (sporadic) to ++++ (extensive).
