**4. Conclusions**

In this work, a systemic infection exclusively originated from *C. glabrata* biofilm cells was simulated and a treatment evaluated. The observations here reported confirmed *C. glabrata* as a low inflammatory species and indicated that two-dose treatment with Csf and Mcf does not have a significant impact on liver and kidney fungal burden or recruited inflammatory infiltrate when mice are i.v. infected with *C. glabrata* biofilm-grown cells.

**Author Contributions:** C.F.R., A.C., M.H., and M.V. conceived and designed the experiments; C.F.R. and A.C. performed the experiments; C.F.R. and A.C. analyzed the data; M.H. and M.V. contributed to the reagents/materials; C.F.R., A.C., M.H., and M.V. wrote the paper.

**Funding:** This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of the Norte2020-Programa Operacional Regional do Norte, financially supported by project UID/EQU/00511/2019—Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE) funded by national funds through FCT/MCTES (PIDDAC), Célia F. Rodrigues' (SFRH/BD/93078/20130) PhD gran<sup>t</sup> and M. Elisa Rodrigues (SFRH/BPD/95401/2013) post-doc grant.

**Acknowledgments:** The authors would like to thank MSD® and Astellas® for the kind donation of Csf and Mcf, respectively.

**Conflicts of Interest:** The authors declare no conflict of interest.
