**5. Conclusions**

According to the results presented here, the most suitable physicochemical, mechanical, and biological properties were presented by Ti6Al4V implants fabricated by selective laser sintering technology, the surface of which was modified by anodization at the 5 V potential, resulting in TNT5 nanoporous coating production. The use of Ti6Al4V/TNT5 and Ti6Al4V/TNT5/AgNPs systems seem to be a promising approach to manufacture implants with anti-inflammatory properties. Both TNT5 and TNT5/AgNPs did not release substances demonstrating mutagenic properties, which is important for the practical use of these materials in implantology. TNT5/AgNPs surfaces also demonstrated the strongest bactericidal and fungicidal activity, most probably thanks to the release of active Ag ions during long-lasting contact with the fluids. It is highly beneficial for implant recipients (i.e. patients) to maintain sterile conditions in the surrounding physiological fluids or tissues after implantation. Finally, mechanical studies proved that both a suitable wear resistance and the ability to propagate energy at plastic deformation during loading characterize this system.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2077-0383/9/2/342/s1, Figure S1: X-ray di ffraction patterns for both sizes of studied Ti6Al4V implants produced using SLS technique, Figure S2: High resolution XPS spectra of TNT5 and TNT15 samples non sputtered and after third sputter.

**Author Contributions:** Conceptualization, P.P. and A.R.; methodology, P.P., A.R., W.J.; formal analysis, P.P., A.R., T.J., M.B., B.S., A.S., Y.K.E.; investigation, P.P., A.R., M.E., T.J., M.B., B.S., B.E., and A.S.; data curation, P.P.; writing—original draft preparation, P.P., A.R., T.J., M.B., B.S., M.W., and A.S.; writing—review and editing, P.P. and A.R.; supervision, P.P.; project administration, P.P. and A.R.; funding acquisition, A.R. and P.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Regional Operational Program of the Kuyavian-Pomeranian Voivodeship (1.3.1. Support for research and development processes in academic enterprises), within the gran<sup>t</sup> obtained by Nano-implant Ltd. The APC was funded by Nano-implant Ltd.

**Acknowledgments:** The authors would like to acknowledge networking support by the COST Action CA16122. The authors would like to thank M.Sc. Marlena Grodzicka for help in carrying out works on the production of TNT/AgNPs systems, Marzena Wi ˛eckowska-Szakiel for help in carrying out microbiological studies and Jakub Piotrowski for help in carrying out immunological tests.

**Conflicts of Interest:** The authors declare no conflict of interest.
