**5. Conclusions**

We designed a less-invasive and faster method to assess burn depth using P-ELISA to determine angiogenin levels from burn blister fluid. This approach is cost-e ffective, easy to use, and potentially accurate. Despite a moderate correlation between conventional plate ELISA and P-ELISA, we demonstrated that P-ELISA is feasible to quantify blister angiogenin levels with distinct results to diagnose SPTB and DPTB wounds. We expect this novel medicine study to pave a possible path toward a simple diagnostic method for assessing burn depth without di fficulty.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2075-4418/10/3/127/s1.

**Author Contributions:** Conceptualization, S.-C.P. and C.-M.C.; data curation, Y.-H.T. and C.-C.C.; formal analysis, Y.-H.T.; funding acquisition, S.-C.P. and C.-M.C.; investigation, Y.-H.T. and C.-C.C.; methodology, Y.-H.T.; project administration, C.-M.C.; resources, S.-C.P. and C.-M.C.; supervision, C.-M.C.; writing–original draft, S.-C.P.; Writing–review and editing, C.-M.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study is funded by research grants from the Ministry of Science and Technology, Taiwan (MOST 107-2628-E-007-001-MY3 to C.-M.C.; MOST 108-2314-B-006-080-MY3 to S.-C.P.), National Cheng Kung University Hospital (NCKUH-10705018), the International Center for Wound Repair and Regeneration at National Cheng Kung University from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan, and by the gran<sup>t</sup> of the Ministry of Science and Technology (MOST 107-3017-F-006-002). and the Frontier Research Center on Fundamental and

Applied Sciences of Matters from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE 107QR001I5) in Taiwan.

**Conflicts of Interest:** The authors declare no conflicts of interest.
