**4. Conclusions**

We have demonstrated an integrated hot embossed PMMA featuring an SMT device for desalting, on-chip extraction, and concentration. The hot embossed PMMA controlling dielectric breakdown enabled nanojunctions' pore size tuning. This method permitted the analysis of aminoglycosides as negatively charged drugs which were off-chip spiked with on-site finger prick blood (~20–100 μL) and labelled with fluorescamine. The nanojunctions created using dielectric breakdown are achieved simply and quickly, and the electric field controlling pore size permits their integration without increasing the fabrication cost. The device allows a simple operation and does not rely on valves or pumps. With integrated reagents (dried fluorescamine and liquid electrolytes) and a hand-held reader, this would create a fully-portable screening device for POC analysis of therapeutics.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2079-6374/9/1/19/s1. Figure S1: Schematic of the PMMA fabrication process. Figure S2: Analysis of Aminoglycosides from whole blood.

**Author Contributions:** Conceptualization, Z.T.A. and M.C.B.; Experiments and data analysis Z.T.A., Y.C.Y. and F.L.; Writing-Original Draft Preparation, Z.T.A.; Writing-Review & Editing, Y.C.Y., F.L. and M.C.B.; Supervision and Project Administration, M.C.B.; Funding Acquisition, M.C.B.

**Funding:** This research was funded by The Australian Research Council (ARC) gran<sup>t</sup> number [FT130100101].

**Acknowledgments:** Z.T.A. would like to acknowledge the Iraqi ministry of higher education and scientific research (MOHESR) and the Iraqi cultural attaché in Canberra for the study funding.

**Conflicts of Interest:** The authors declare no conflict of interest.
