*5.4. Statistical Analysis*

The reported values on viability are expressed as the means ± the standard error of the mean (SEM) of at least three biological replicates, with duplicate wells for each dose. The IC50 value, the dose of each toxin leading to 50% viability, was determined using CompuSyn statistical software (CompuSyn Version-1 Inc. Paramus, NJ, USA). Significant differences between groups were analyzed using the Bonferroni multiple comparison test in GraphPad (GraphPad Prism 4 La Jolla, CA, USA).

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2072-6651/11/12/727/s1, Figure S1: Worldwide contamination of emerging mycotoxins. Figure S2: Abundance of emerging mycotoxins in finished pig feed. Figure S3: Scatter plot of the correlation between DON and 10 selected emerging mycotoxins. Figure S4: Dose-effect curves of the individual toxicity of DON and 9 selected fungal emerging mycotoxins Table S1: Concentration of the metabolites in 3 quartiles (P25, P50 and P75) and ratios between the concentration of DON and 10 selected emerging mycotoxins. Figure S5: LC-ESI(+)-MRM chromatograms of one pig feed sample. Figure S6: Overlay of the extracted ion chromatograms of both quantifier as well as qualifier of the 11 investigated compounds in one pig feed sample.

**Author Contributions:** A.K.K., I.P.O. and P.P. designed the experiment, A.K.K. and M.N. performed the experiments, B.N. and G.S. contributed to provide raw data on finished pig feed, T.J. analyzed raw data on finished pig feed, P.G.P.M. performed statistical analysis on correlation, A.K.K. analyzed the cytotoxicity results, A.K.K., P.P. and I.P.O. wrote the paper, B.N. and P.G.P.M. reviewed the paper.

**Funding:** This research was supported by the ANR grants "EmergingMyco" (ANR-18-CE34-0014) and "Newmyco" (ANR-15-CE21-0010) and a grant from BIOMIN. A.K.K was partially supported by the regional contract 15066653.

**Acknowledgments:** We would like to thank Terciolo for her critical review of the paper and Daphne Goodfellow for English editing.

**Conflicts of Interest:** B.N., T.J. and G.S. are employed by BIOMIN. However, this circumstance did not influence the design of the experimental studies or bias the presentation and interpretation of results. The other authors, A.K.K., P.G.P.P., M.N., P.P. and I.P.O. declare no conflict of interest.

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