*5.6. Detection of Inflammatory Mediators and Intestinal Permeability Indicators*

The cells were treated with DON and PDTC, and then cultured in an incubator for 24 h. The culture solution was centrifuged at 1000 rpmmin−<sup>1</sup> for 5 min to obtain a cell culture supernatant. The cells were collected in a cell via a subculture method, and the cell lysate was collected. The NO, IL-6, and TNF-α in the cell lysate were used according to the method of the ELISA kit (Senbeijia Biological Technology, Nanjing, China). The activity and the DAO in the cell culture supernatant was measured, and its activity was calculated according to a self-drawn standard curve.
