**4. Conclusions**

Halloysite or montmorillonite were loaded in an electrospun polysaccharidic scaffold in a one-pot process. Halloysite scaffolds were characterized by a fiber regular structure and smooth surface, and did not show any structural alterations when embedded in the polymeric matrix, probably due to the nanotubular structure of this clay mineral. MMT scaffolds were characterized by nanofiber portions with a regular, smooth surface, spaced out in broadened parts as knots with a scattered structure, possibly due to its structure. Moreover, MMT inclusion in the polymeric matrix of the scaffold caused interlayer space enlargement, causing the biopolymer intercalation into the MMT galleries, resulting in a deep interaction between the scaffold matrix and clay mineral. HNT or MMT (2% concentration) in the scaffolds were able to sustain homogeneous fibroblast spreading all over the scaffolds and their growth up to confluency, maintaining a cell fusiform structure and aligned and elongated cytoskeleton filaments. HNT and MMT (2% concentration) scaffolds. Due to their capability to support and enhance fibroblasts proliferation with negligible proinflammatory activity, these scaffolds are promising for applications in wound healing: Their capability to enable cell attachmnent and adhesion is probably due to their morphological 3D structure-assisted cell homing, and this could facilitate wound healing in vivo.

#### **5. Patents**

Sandri: G.: Bonferoni, M.C.; Rossi, S.; Ferrari, F. Electrospun nanofibers and membranes, PCT/IT2017/000160, 2017.

**Author Contributions:** Conceptualization, G.S., A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response); methodology, G.S.; A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response); software, F.F. and M.C.B.; validation, C.V.; investigation, M.L., A.F., D.M., M.R.; data curation, G.S., C.A. A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response); writing—original draft preparation, G.S., M.R., A.F.; writing—review and editing, G.S., A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response); supervision, G.S., A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response); project administration, G.S.; funding acquisition, G.S., S.R., F.F., M.C.B., A.P.-M. (Cytocompatibility of Macrophages and Pro-inflammatory Immune Response). All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was partially supported by Horizon 2020 Research and Innovation Programme under Grant Agreement No. 814607.

**Acknowledgments:** The authors wish to thank Giusto Faravelli SpA for supplying the polymers.

**Conflicts of Interest:** The authors declare no conflict of interest.
