2.2.3. Preparation of Electrospun Scaffolds

Scaffolds were obtained using an electrospinning apparatus (STKIT-40, Linari Engineering, Italy), equipped with a high-voltage power supply (40 kV), a volumetric pump (Razel R99-E), a 10 mL syringe, and a conductive static collector, covered by aluminum foil. The following parameters were used: ΔV (voltage) = 22 kV, collector spinneret distance = 24 cm, polymeric solution flow = 0.4 mL/h, spinning time = 1.30 h, temperature = 30 ◦C, relative humidity = 30%; and needle dimensions: 0.5 × 20 mm for MMT and 0.4 × 20 mm for HNT. The obtained scaffolds were then crosslinked by heating at 150 ◦C for 1 h, to prevent their solubilization in aqueous media and to allow cell homing. The heating process is also reported as able to dry sterilize the products [17].
