*3.3. Biocompatibility of ALIG30@20*

Biocompatibility results of ALIPS9@20 are reported in Figure 2. As previously mentioned, G30 and ALIG30@10 results have already been assessed by García-Villén et al. [17]. The reduction in viability produced by the pristine G30 alone at 1 mg/mL was not found in any of the hydrogels. In fact, the viability results of ALIG30@20 demonstrated, once again, that the type of formulation exerts a significant role in the results. That is, despite all tests subjected to the same amount of clay mineral in the culture, the hydrogels increased the biocompatibility. In particular, ALIG30@20 showed cellular viabilities higher than 100% at every concentration (*p* > 0.05 with respect to GM, Figure 2). In view of the experimental results and the statistical analysis, it is possible to state that ALIG30@20 exerts proliferative effects over fibroblasts at the tested concentrations. No other internal statistical differences were found between ALIG30@20 concentrations.

**Figure 2.** Biocompatibility tests of ALIG30@20 (blue). Viability (%) vs. hydrogel or clay concentration (% w/w). GM (growth medium) indicates the control. G30 and ALIG30@10 results (taken from García-Villén et al. [17]) were included to compare viability results of hydrogels with different concentrations. Mean values ± s.e.; *n* = 8. Significant differences, compared to GM, are marked with (\*). Mann–Whitney (Wilcoxon) W tests, *p* values ≤ 0.05.
