*3.6. Allogenic Circulating B-Cell and T-Cell Assays*

Peripheral circulating donor HLA-specific memory B cells quantified by enzyme-linked immunospot (ELISPOT) [77] and serum B-cell activating factor level on post-operative day 7 [78] both predicted ABMR, especially in DSA-positive recipients.

Pre-transplant T cell alloreactivity can be assessed with a donor-specific IFN-γ ELISPOT which measures IFN-γ release by recipient T cells in response to donor antigens. IFN-γ ELISPOT intensity appears to correlate with development of subclinical TCMR, ABMR, and DSA [79,80].
