*4.3. Experimental UUO Model*

After anesthesia, the UUO model was performed in mice by ligation of the left ureter with 5-O silk through a flank incision, as previously described [26]. After the surgery, the mice were recovered under a warming lamp. For HDAC inhibition experiments, TSA (1 mg/kg, Sigma-Aldrich) or the vehicle were injected intraperitoneally daily. The animals were euthanized, and the obstructed kidneys were harvested 7 and 14 days after UUO for further analyses.
