*4.4. Protein Search and Label-Free Quantification*

The raw data files were processed by MaxQuant 1.6 software (Max Planck Institute for Biochemistry, Martinsried, Germany) [31]. Label-free quantification (LFQ) parameter was enabled. Unique and razor peptides were chosen for protein quantification. Carbamidomethylation of cysteine was set as a fixed modification, and oxidation of methionine and N-term acetylation were set as variable modifications. Protein search was performed against an actual SwissProt protein database with the human taxonomy restriction. The Orbitrap instrument was chosen and mass tolerance of 20 ppm was set for the first search and 4.5 ppm was set for the main search. Protein false discovery rate (FDR) of 0.01 was set. The obtained values of LFQ intensities (Table S1) were considered as relative indicators of protein expressions over the sample groups.
