*2.1. FX Decreased White Adipose Weight without Altering Food Intake*

Four groups of mice were respectively fed the 4 test diets shown in Table 1. The results of two-way ANOVA on the four groups (Table 2) of mice showed that neither FX nor diet affected final body weight, body weight gain and energy efficiency (*p* <sup>&</sup>gt; 0.05). The high fat diet, but not FX, decreased food and energy intake (*p* < 0.05) (Table 2). FX significantly decreased relative weight of both abdominal, eWAT and retroperitoneal WAT (rWAT), and subcutaneous iWAT (*p* < 0.001). High fat diet decreased, but FX increased, the relative weight of BAT, (*p* < 0.05) (Table 3). Liver,  kidney, spleen and heart relative weights were significantly increased by FX (Supplementary Table S1). 

**Table 1.** Composition of the 4 test diets: HS, high sucrose diet; HS + F, high sucrose diet supplemented with fucoxanthin; HF, high fat diet; HF + F, high fat diet supplemented 

with fucoxanthin. The composition of vitamin mix and mineral mix are in accordance with AIN-93 and AIN-93G, respectively [37]. 


**Table 2.** Initial body weight, final body weight, body weight gain, food/energy intake and energy efficiency of C57BL/6J male mice fed test diets for five weeks. Values are the means ± SD ( *<sup>n</sup>* = 4). \* denotes significant influence by either dietary factor at *p* < 0.05 analyzed by twoway ANOVA. Energy efficiency = grams of body weight gain/1000 kcal energy intake. FX, fucoxanthin. 


**Table 3.** Relative tissue weights (percent of body weight) of C57BL/6J male mice fed test diets for five weeks. Values are the means ± SD ( *<sup>n</sup>* = 4). \* denotes significant influence by either dietary factor at *p* < 0.05 analyzed by two-way ANOVA. iWAT, inguinal white adipose tissue (WAT); eWAT, epididymal WAT; rWAT, retroperitoneal WAT; BAT, brown adipose tissue.

