*3.4. Tissue and Serum Collection*

After feeding test diets for 5 weeks, mice were deprived of diet for 12 h and killed by CO2 asphyxiation. Blood was withdrawn through retro-orbital. Serum was obtained after centrifugation at 12,000 rpm for 10 min at 4 °C and stored at ƺ80 °C. Liver, spleen, kidney, heart, lung, gastrocnemius muscle, retroperitoneal WAT (rWAT), eWAT, inguinal (iWAT) and intra-scapular BAT were excised, weighed and immediately frozen in liquid nitrogen and stored at ƺ80 °C for mRNA analysis. 
