*3.1. Materials*

Picryl chloride (PCl; 2,4,6-trinitrochlorobenzene) was purchased from Nacalai Tesque (Kyoto, Japan). Cyclophosphamide was purchased from Shionogi Pharmaceutical Co. (Osaka, Japan). Hydrocortisone was purchased from Sigma-Aldrich (St. Louis, MO, USA). Recombinant mouse eotaxin (CCL11) was purchased from ProSpec-Tany TechnoGene Ltd. (Ness Ziona, Israel). Peridinin and fucoxanthin were isolated from a dinoflagellate, *Symbiodinium* sp. (OTCL2A strain), and brown alga, *Petalonia fascia*, in culture, respectively. 

*3.2. Preparation of Peridinin* 

Peridinin was isolated from a cultured dinoflagellate, *Symbiodinium* sp. (OTCL2A strain). This *Symbiodinium* sp. was isolated from the giant clam, *Tridacna crocea*, by the author and cultured by a method reported previously [2]. The frozen cells (82.0 g from 132 L of culture) were homogenized in 70% EtOH (150 mL) with an Ultra-Turrax T25 homogenizer (Janke & Kunkel GmbH & Co. KG IKA-Labortechnik, Staufen, Germany), soaked for 3 days at 4 °C and centrifuged. The supernatant was collected; the precipitate was extracted twice with 70% EtOH (150 mL each). The ethanol extracts from the centrifugation and extractions were combined and evaporated *in vacuo*. The residue was suspended in water (100 mL) and extracted with EtOAc (3 × 200 mL). The combined EtOAc extracts were concentrated under reduced pressure and yielded an EtOAc-soluble fraction (903 mg). A portion (676 mg) of the EtOAc-soluble fraction was chromatographed on silica gel (60 mL of Silica Gel 60, Nacalai Tesque) and eluted with 120 mL of CH2Cl2:MeOH in fractions on a gradient of 99:1, 98:2, 96:4 and 92:8. A portion (199.2 mg) eluted from the last two extractions with CH2Cl2:MeOH (96:4 and 92:8; 264.8 mg) was then separated by chromatography on ODS (19 mL of Cosmosil 75C18-OPN, Nacalai Tesque) followed  by eluting steps with 40 mL of 80% MeOH and 80 mL of 85% MeOH. The fraction eluted with 85% MeOH had a crude yield of 98.8 mg and, following separation by HPLC (COSMOSIL 5C18-AR-II, 20 mm Π × 250 mm, Nacalai Tesque) with 85% MeCN at a flow rate of .0 mL minƺ1, yielded peridinin (24.7 mg). 
