2.4.2. HSV-1

MJS cells (10<sup>5</sup>/well) were seeded in a 24-well plate and cultured overnight at 37 ◦C. HSV-1-eGFP was mixed with CPXV012 peptide, control peptide UL49.5, or DMSO only in complete medium. DMSO concentration was 0.1% with or without peptide. The medium of cells was replaced with virus/peptide inoculum (MOI 0.1) and cells were incubated for 16 h at 37 ◦C. Cells were harvested, fixed using 1% formaldehyde, and the number of eGFP-positive cells was determined using flow cytometry.
