*2.9. Immunofluorescence*

For assessment of viral proteins, immunofluorescence was carried out as described [13]. Briefly, cells were fixed with 2% ( *w*/*v*) paraformaldehyde in PBS and permeabilized with 0.1 Triton X-100 followed by incubation with mAb anti-E1 from Viral Antigens (Viral Antigens Incorporation, Memphis, TN, USA) at a 1:200 dilution as primary antibody.

### *2.10. Statistical Analysis*

All statistical calculations were done with Graph Pad Prism software (GraphPad Software, Inc., La Jolla, CA, USA). Asterisks (\* *p* < 0.05, \*\* *p* < 0.01, \*\*\* *p* < 0.001, \*\*\*\* *p* < 0.0001) highlight the level of significance in diagrams which include data as means ± standard deviation (SD). For comparison of normalized mRNA expression levels in RV-infected samples with the corresponding mock controls, a paired Student's *t* test (consistent ratios of paired values) was applied. Statistical analysis for di fferent samples was based on one-way ANOVA followed by Bonferroni's multiple comparison test.
