**5. Conclusions**

In summary, the EPS 26a produced by *Lactobacillus* sp. possessed significant anti-HAdV-5 activity, based on the obstruction of HAdV-5 reproduction, inducing the formation of non-infectious virus progeny. Thus, this LAB strain is a promising producer of EPS with antiviral activity against HAdV-5. However, further investigation is needed to explore the antiviral mechanism of such an EPS in detail. **Author Contributions:** Conceptualization, L.B. and Y.P.; Funding acquisition, L.B.; Investigation, L.B., Y.P., O.P.; Methodology, S.Z. and L.B; Resources, S.Z. and L.B.; Visualization, L.B.; Writing-original draft, L.B.; Writing—review & editing, O.P. and S.Z.

**Funding:** This research was funded by the State Fund for Fundamental Research of Ukraine (grant number F83).

**Acknowledgments:** We thank Vasyliuk O.M. and Garmasheva I.L. from the department of physiology of industrial microorganisms of the Danylo Zabolotny Institute of Microbiology and Virology NASU for the provided exopolysaccharides used in the study.

**Conflicts of Interest:** The authors declare no conflict of interest.
