**4. Conclusions**

In general, the four hydroponic *Brassicaceae* microgreens produced in this study could be considered as good sources of minerals and antioxidant phytochemicals in a balanced human diet. In particular, they contain relevant amounts of vitamin C, higher levels of total carotenoids than adult plants, mineral and antioxidant bioactive compound contents comparable to other hydroponic microgreens, and low K, making them suitable for patients with impaired kidney disease.

In this study, bioaccessibility data for antioxidant bioactive compounds, total antioxidant capacity, and mineral elements in microgreens are provided for the first time. Radish and mustard showed the highest BF and BA values for antioxidant parameters, while broccoli and mustard provided the lowest and highest values for minerals, respectively. Despite the expected decrease in different compounds after the in vitro digestion process, the bioaccessible fractions of microgreens still contained remarkable total antioxidant capacities and bioactive compounds with potential beneficial local effects in the gastrointestinal tract. For future studies, determining the bioaccessibility of the antioxidant phytochemicals in more microgreen species, as well as their potential bioactivity in pre-clinical and human intervention studies, ought to be addressed.

**Author Contributions:** Conceptualization and funding acquisition: A.C., R.B., and A.A.; methodology: A.C., R.B., and A.A.; analysis and experiments: B.F. and G.L.-G.; writing—review and editing: B.de la F., G.L.-G., V.M., A.C., R.B., and A.A.

**Funding:** This research was funded by the University of Valencia through the project OTR2017-17285INVES, supported by CIAM\_Grupo Alimentario Citrus, in which Beatriz de la Fuente was contracted.

**Acknowledgments:** The authors want to express their acknowledgment to the technical support from the atomic spectroscopy section from the Servicio Central de Soporte a la Investigación (SCSIE) at the University of Valencia for the help in the analysis of minerals.

**Conflicts of Interest:** The authors declare no conflict of interest.
