*2.2. Reagents*

#### 2.2.1. In Vitro Gastrointestinal Digestion

Pepsin (porcine, 975 units per mg protein), pancreatin (porcine, activity equivalent to 8 × USP specifications), bile extract (porcine), ammonium carbonate ((NH4)2CO3), sodium bicarbonate (NaHCO3) and calcium chloride dihydrate (CaCl2(H2O)2) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Potassium chloride (KCl), sodium chloride (NaCl), magnesium chloride hexahydrate (MgCl2(H2O)6) and potassium dihydrogen phosphate (KH2PO4) were supplied by Merck (Darmstadt, Germany). Culture-grade water was obtained from B. Braun (Melsungen AG, Germany). Simulated Salivary Fluid (SSF), Simulated Gastric Fluid (SGF), Simulated Intestinal Fluid (SIF) and enzymatic activity assays were prepared according to Minekus et al. (2014). A water bath with orbital shaking (Stuart SBS30, Sta ffordshire, UK) and centrifuge (Eppendorf 5810, Hamburg, Germany) were used to simulate the gastrointestinal digestion process.

#### 2.2.2. Bioactive Compounds and Antioxidant Capacity

Glacial acetic acid, metaphosphoric acid, formic acid and L (+) ascorbic acid (≥99%) were supplied by Panreac Química (Barcelona, Spain). Sodium salt dihydrate 2,6-dichlorophenolindophenol (DCFI), Tris(hydroxymethyl)aminomethane, potassium phosphate monobasic (Na2HPO4), potassium phosphate dibasic (K2HPO4), sodium phosphate dibasic (Na2HPO4), potassium chloride (KCl) and sodium acetate (C2H3NaO2) were obtained from Merck (Darmstadt, Germany). Sodium carbonate (Na2CO3), potassium persulfate (K2S2O8), Folin-Ciocalteu reagent, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), gallic acid and sulforaphane standard (≥90%) were purchased by Sigma Chemical Co. (St. Louis, MO, USA). Ethanol (96%), methanol and n-hexane (96%) were provided from Scharlau (Barcelona, Spain). Sodium fluorescein was obtained from Fluka Chemie AG (Bunds, Switzerland) and 1,2-benzenedithiol (BDT) (96%) from Acros organics (BVBA Thermo Scientific, Geel, Belgium). Water was purified by a Milli-Q system (Milford, MA, USA).
