**4. Conclusions**

Germination of lentil, fenugreek, alfalfa, and daikon radish led to the accumulation of their total polyamines. A large increase in CAD content was observed in all three legume sprouts. In the microgreens of these legumes, the CAD content was substantially reduced, whereas here, compared to the sprouts, more AGM, SPD, or SPM accumulate. These are considered to be nutritionally beneficial. In daikon radish sprouts, AGM is the major polyamine, while the formation of microgreens led to a reduction in the content of the nutritionally beneficial polyamines. This behavior was reversed with respect to the changes in the content in legumes.

Tissue damage of legume sprouts led to considerable degradation of polyamines, especially PUT, CAD, and AGM. Experimental artifacts or modulation of the polyamine composition of sprouts are among the possible implications. Homogenized fenugreek sprouts can be used for the degradation of exogenous PUT, CAD, and TYR at pH-values above 5.

**Author Contributions:** I.K.C. and B.C. conceived and designed the experiments and supervised experimental work; S.R. and T.R. performed experiments and analyzed the data. All authors, including N.P.U., participated in writing and revising this article. All authors have read and agreed to the published version of the manuscript.

**Funding:** The work was financially supported by the research programs funded by the Slovenian Research Agency (P4-0121 and P1-0153).

**Acknowledgments:** The authors gratefully acknowledge Roger H. Pain and Robert Susiˇc for their critical reading and appraisal of the manuscript and Anja Kavˇciˇc for her help and advice on the growth of microgreens.

**Conflicts of Interest:** The authors declare no conflict of interest.
