2.2.1. AMsil1

AMsil1 concentration did not exert a statistically significant effect on the viability of CCL1 cells (Figure 2). However, the exposure time reduced the number (1.2-fold difference of mean, *p* ≤ 0.002) of live cells. Similarly, AMsil1 concentration did not affect the metabolic activity of CCL1 cells. Time of exposure had a modest effect (*p* ≤ 0.02) on CCL1 metabolic activity, although no significant paired comparisons (within each concentration) were observed.

**Figure 2.** Percent control value of viability (**a**) and metabolic activity (**b**) of CCL1 cells exposed to 2-fold serial dilutions of AMsil1 (≤8.34 mmol/L) for 24 or 72 h. Data represent mean ± standard error for five independent replicates tested in triplicate.

Independent of time, AMsil1 concentration exerted a main effect (*p* ≤ 0.001) on the number of viable HGFs (Figure 3). Paired comparisons indicated that at lower AMsil1 concentrations (≤0.13 mmol/L), the number of live HGFs decreased. Independent of AMsil1 concentration, exposure time reduced (19.73 difference of means, *p* ≤ 0.001) HGF viability. Monomer concentration or time of exposure did not statistically affect the metabolic activity of HGF cells (data not shown).

**Figure 3.** Percent control value of viability of human gingival fibroblast (HGF) cells exposed to 2-fold serial dilutions of AMsil1 (≤8.34 mmol/L) for (**a**) 24 h or (**b**) 72 h. Data represent mean ± standard error for five independent replicates tested in triplicate.
