*3.2. Bioassays*

Table 1 shows the different percentages of growth inhibition of *L. sativa* in three samples for five evaluated stations. As the results show, there was root inhibition in some sampling stations and overgrowth in others. In Village Gato, in the third sampling, there was excess growth at a concentration of 25%. Likewise, in Village Tigui, the greatest inhibitions registered were observed at a concentration of 25%. In general, among all the sampled stations where the greatest inhibition was observed the highest was in Village Tigui, followed by house, deep-well underground, Village Gato, and the smallest recorded in the water catchment of the Boque River.



S: sampling, the numbers 1S, 2S, and 3S correspond to the months of July, September, and December in which the sample was taken; n: is the number of samples.

In Table 2, it is observed that the *D. magna* indicator has different mortality percentages since the same four concentrations of the sample are evaluated as in the *L. sativa* bioassay. The highest was 23% mortality at a concentration of 50% at the water catchment of the Boque River station and 17% mortality at 75% at the Village Tigui sampling station, followed by the house and deep-well underground stations. Finally, the lowest concentration of mortality was obtained in the Village Gato station with 33% mortality at 100%. For *H. attenuata*, it was not possible to determine the EC50 and LC50 values because there were no morphological changes, indicating lethality or sublethality, in the three samplings carried out, reporting 0% sublethality at 100% (*v*/*v*) and 0% lethality at 100% (*v*/*v*).
