*2.4. Assay of Antioxidant Enzymes Activities*

*Bellamya unicolor* tissues were separated from the shell and washed with deionized water; 0.25 g of the tissue sample was then homogenized using 0.01 (M) chilled phosphate buffer at pH 7 with ice-cold mortar and pestle. The homogenate was later centrifuged for 25 min at 4 ◦C and 14,000 rpm [1,28]. The supernatant from the centrifuged samples was used for the measurement of antioxidant enzyme activities using an LT-291 Single Beam ultraviolet-visible (UV-VIS) spectrophotometer.
