2.2.5. Freeze Drying of Itraconazole (ITR)

Firstly, a 10 mg/mL solution of ITR in dioxane was prepared by weighing 1 g of the API and solubilizing it in 100 mL of the solvent. The solution was divided into three round-bottomed flasks and frozen using liquid nitrogen while rotating using a Rotavapor R-205 (Büchi, Flawil, Switzerland). The samples were dried for 18 h using a freeze drier, ALPHA 2-4 LSC (Martin Christ, Osterode am Harz, Germany), with manifolds for a connection of NS 29/32 flasks under a vacuum of 2 × 10−<sup>3</sup> mbar, between 5 and 6 m<sup>3</sup>/<sup>h</sup> suction and ice condenser adjusted to −85 ◦C. No secondary drying step was applied.
