2.2.3. Particle Size Analysis

The mean PS and PDI of NCC was determined using a Nano-ZS 90 Zetasizer (Malvern Instruments, Worcestershire, UK) with the instrument set in Photon Correlation Spectroscopy (PCS) mode. Approximately 30 μL of an aqueous dispersion of NCC was diluted with 10 mL HPLC-grade water prior to analysis. The sample was placed into a 10 × 10 × 45 mm polystyrene cell and all measurements were performed in replicate (*n* = 6) at 25 ◦C using a standard 4 mW laser set at 633 nm at a scattering angle of 90◦. Analysis of PCS data was undertaken using Mie theory with real and imaginary refractive indices set at 1.456 and 0.01.
