*2.3. Cytotoxicity*

Figure 4 shows the fibroblast L929 cells viability in view of different concentrations of silver nanoparticles (green and conventional route). Green silver nanoparticles presented lower cytotoxicity than conventional ones. A dosage of 50 μg/mL was necessary to initiate the toxicity, but the

cell viability was nearly 80%, while conventional-silver nanoparticles were quite toxic at very low concentration (6.25 μg/mL) and was similar to the negative control (DMSO) with viability lower than 20%. Furthermore, the addition of the reagents to prepare the formulations did not interfere in the toxicity of the conventional-silver nanoparticles, whereas the cytotoxicity for the green-silver nanoparticles formulation as well as for the extract formulation was considerably increased.

**Figure 4.** Cytotoxicity evaluation of respective active input (green and chemical silver nanoparticles and pomegranate peel extract), their respective formulations, and the vehicle (compounds of spray-formulation without the active inputs). (**A**) Silver nanoparticles green; (**B**) Silver nanoparticles green formulation; (**C**) Silver nanoparticles chemical; (**D**) Silver nanoparticles chemical formulation; (**E**) Pomegranate peel extract; (**F**) Pomegranate peel extract formulation and (**G**) Vehicle.
