**5. Conclusions**

In light of the results obtained and the limitations of the present study, it was concluded that the use of pomegranate peel extract showed it to be an efficient reducing agen<sup>t</sup> for the production of silver nanoparticles. Moreover, the antimicrobial potential and the low cytotoxicity demonstrated by green-silver nanoparticles have stimulated the search for improvements in the bio-nanotechnology field. Furthermore, the anti-inflammatory and antioxidant properties of pomegranate have encouraged further studies to use nanosystems with future application in prophylaxis or treatment of biofilm-dependent diseases.

**Author Contributions:** R.A.F., D.B.B., and A.A.B. conceived and designed the experiments; R.A.F., E.C.T., A.F.M.B., G.L.F., and C.C.M.-G. performed the experiments; R.A.F., D.B.B., and A.A.B. analyzed the data; F.N. d.S.-N., L.F.G., and E.R.d.C. contributed reagents/materials/analysis tools; R.A.F. and D.B.B. wrote the paper.

**Funding:** This study was supported by São Paulo Research Foundation (FAPESP), Brazil, (Process n<sup>º</sup> 2016/04230-9).

**Acknowledgments:** The authors would like to thank the company Apis Flora Indl. Coml. Ltda. for the facilities and for the production of the spray formulations containing pomegranate peel extract, and the Laboratory of photochemioprotection of the Faculty of Pharmaceutical Sciences of Ribeirão Preto for the facilities in performing some of the laboratory assays. We thank the Brazilian Agricultural Research Corporation (EMBRAPA) to allow for some tests on the research on its premises and also the Federal University of São Carlos for disposal of its dependencies and technologies. This research work was supported by the São Paulo Research Foundation (FAPESP), Brazil, (Process n<sup>º</sup> 2016/04230-9).

**Conflicts of Interest:** The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
