**4. Conclusions**

Fabricated Ag NPs using a rapid, one-step green approach, based on microwave irradiation showed desirable physico-chemical properties with antibacterial activity against *E. coli* and *S. aureus*. However, their antibacterial activity was significantly (*p* < 0.05) higher toward Gram-positive bacteria strains. The synthesized Ag NPs using the rapid developed synthesis method can be used as a favorable antibacterial agen<sup>t</sup> in different areas such as medicine and food packaging.

**Author Contributions:** M.E. performed the Ag NPs synthesis and physico-chemical analyses. H.V. performed strain cultivation and antibacterial assay. Y.N. and M.J.N. contributed with valuable discussions and scientific advice. H.J.-M. designed the experiments and wrote the draft manuscript. A.B. contributed helpful discussion and statistical analysis during the preparation of manuscript.

**Funding:** This research received no external funding.

**Acknowledgments:** This research was undertaken with material support from the Najian Herbal Group (Tabriz, Iran). The authors appreciate this support. This research received no specific gran<sup>t</sup> from any funding agency in the public, commercial, or not-for-profit sectors.

**Conflicts of Interest:** The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of this article.
