**5. Conclusions**

Soy isoflavones nanoparticles using polymerized goa<sup>t</sup> milk whey protein as wall material were prepared and characterized in this study. The results suggested that polymerized goa<sup>t</sup> milk whey protein prepared directly from milk was suitable to encapsulate soy isoflavones with high encapsulation efficiency, and hydrophobic interaction was considered to be the main force in the formation of the nanoparticles. The findings will be helpful for the use of polymerized goa<sup>t</sup> milk whey protein as a carrier material for hydrophobic bioactive compounds.

**Author Contributions:** M.T.: conceptualization, formal analysis, investigation, resources and writing—original draft; C.W.: formal analysis, investigation, resources and visualization; J.C.: formal analysis and resources; H.W.: formal analysis, investigation, resources and visualization; S.J.: formal analysis and resources; M.G.: conceptualization, funding acquisition, project administration, writing—original draft and writing—review & editing. All authors approved the final version of the manuscript.

**Funding:** The Project was supported by the Millions of Engineering Science and Technology Major Special Program of Heilongjiang Province (Contract No. 2019ZX07B01).

**Conflicts of Interest:** The authors declare no conflict of interest.
