*2.5. Statistical Analysis*

Data were tested for normality using the Shapiro–Wilk normality test. For normal distributions, means and standard deviations were calculated, and for non-normally distributed data, medians and ranges were calculated. Due to the scarcity of information on tiger electrophoretic patterns, values were compared to cheetah [13] and domestic cat [17] reference values. Due to the small sample size, the reference interval (RI) limits are directly estimated by the minimum and maximum values [26]. Statistical analysis was performed using MedCalc Statistical Software version 15.11.3 (MedCalc Software, Ostend 8400, Belgium).
