**Ejaculate Collection Influences the Salivary Oxytocin Concentrations in Breeding Male Pigs**

**Marina <sup>L</sup>ópez-Arjona 1, Lorena Padilla 2,3, Jordi Roca 2,3, José Joaquín Cerón 1,\* and Silvia Martínez-Subiela 1**


Received: 24 June 2020; Accepted: 16 July 2020; Published: 25 July 2020

**Simple Summary:** This study aimed to evaluate how the process of ejaculate collection a ffects oxytocin concentrations in saliva of boars used in artificial insemination. Saliva samples of 33 boars were collected the day before ejaculate collection, during the ejaculation time, and two hours after ejaculate collection. Free oxytocin and oxytocin linked to proteins were quantified in these saliva samples. Oxytocin concentrations during the ejaculation time were higher than the day before with oxytocin linked to proteins showing higher di fferences. In addition, younger boars, boars with higher libido intensity and boars of the Pietrain breed showed higher values of oxytocin in saliva during ejaculation than the day before. This study demonstrated that ejaculation influences the salivary oxytocin concentrations boars.

**Abstract:** The objective of the present study was to evaluate the possible changes of oxytocin concentrations in saliva during and after ejaculate collection in breeding boars usually used in artificial insemination programs. Saliva samples of 33 boars were collected the day before ejaculate collection (DB), during the ejaculation time (T0) and two hours after ejaculate collection (T2). Free oxytocin and oxytocin linked to proteins concentrations were measured by two methods previously developed and validated for saliva of pigs. Younger boars, boars with higher libido intensity and boars of the Pietrain breed showed higher values of oxytocin in saliva during ejaculation than the day before. In addition, boars with higher libido showed higher concentrations two hours after ejaculate collection than during the day before. These changes were of higher magnitude and significance when oxytocin linked to proteins was measured. In conclusion, this study demonstrated for the first time that ejaculation influences the salivary oxytocin concentrations in breeding boars, although this influence varies according to age, libido and breed.

**Keywords:** oxytocin; boar; saliva; ejaculation
