*2.3. Image Analysis*

All images were analysed using FLIR Tools version 5.13 software for extraction of thermal metrics. The FLIR Tools software enabled us to measure the temperature of specifically selected body features of a koala by simply defining them within each image (Figure 1). We were most interested in measuring the temperature of the eyes, ears, paws, abdomen, and back.

The coefficient of variation (the ratio of the standard deviation to the mean) was calculated for each body feature (eyes, ears, paws, abdomen, or back) to determine which was the most consistent for measuring body temperature using a thermal imaging camera, i.e., the body features with the least amount of variation.
