*2.8. Statistical Analysis*

The total number of cells were counted manually in a defined fixed quadrant and equal area of each images. Average number of the cells were accounted for further the descriptive statistical analysis included mean and standard deviation (SD) described in Section 2.6. *t*-Test was used to calculate significance for qPCR data and mean fold changes ± SE were plotted in a graph. A *p*-value less than 0.05 was taken as statistically significant.
