*2.9. Statistical Analysis*

For quantitative variables, and after assessing application conditions, all parameters were subjected to the one-way analysis of variance (ANOVA) followed by the Bonferroni post-hoc test. All groups were normalized by each pass and compared versus a control group. Data are expressed as mean ± SEM. A di fference *p* < 0.05 was considered statistically significant. GraphPad Prism 6.0 (GraphPad Prism Software Inc., San Diego, CA, USA) was used for statistical analysis.
