*4.6. Cell Cycle Analysis*

Following treatment with different concentrations of multifloroside for 48 h, the distribution of cell cycle was measured using the cell cycle detection kit according to the manufacturer's instructions. The cell cycle of the treated cells was then detected by flow cytometry to quantify the amount of DNA in each cell (ACEA NovoCyte™, ACEA Biosciences, San Diego, CA, USA).
