4.5.1. Biochemical Analysis

The collected blood samples were separately allowed to clot and centrifuged at 1200 rpm for 10 min. Serum was collected and used for estimation of some selected biochemical parameters. The serum levels of total antioxidant status, GSH, SOD, XO, and MDA were estimated using commercial activity assay kits (Sigma Aldrich, St. Louis, MO, USA). The total antioxidant status assay kit non-specifically measures the total concentration of the antioxidant molecules (small molecules and proteins) in a biological sample (serum, cell and tissue lysate etc.). The serum IL-6 content was measured with ELISA kit (Sigma Aldrich, St. Louis, MO, USA). Standard laboratory procedures (Global Clinical & Viral Laboratory, Kwazulu Natal, South Africa) were followed to estimate the serum levels of Cr, UA, ACE, and BUN.
