*3.7. ABTS Radical Cation Scavenging Activity*

Each extract sample was diluted 10-fold with water, then the ABTS value was evaluated as described by Re et al. [25]. In brief, ABTS was measured using pre-formed radical monocations. The mixtures, along with 7.4 mM ABTS solution and 2.6 mM potassium persulfate, were incubated at room temperature in the dark for 24 h. The ABTS solution was diluted with phosphate-buffered saline (pH 7.4) to achieve an absorbance of 0.7 ± 0.02 at 734 nm. Each sample (10 μL) was then reacted with 190 μL of the ABTS solution. The absorbance at 734 nm was measured 6 min after the reaction using the Benchmark Plus ELISA reader (Bio-Rad, Hercules, CA, USA).
