4.3.2. Carrageenan-Induced Peritonitis

The mice (*n* = 6) were orally pre-treated with the vehicle, NTK (10 mg/kg), or indomethacin (25 mg/kg) 1 h before receiving an intraperitoneal injection of 1 mL of 1% carrageenan (challenge). Four hours after challenge, these animals were euthanized by CO2 inhalation, the peritoneal cavity was washed with 3 mL of heparinized PBS (10 IU/mL), and the peritoneal lavage was collected for leukocyte counting and quantification of MPO and total proteins [26]. Total leukocyte counts were performed using the SDH-20 model automatic counter and the di fferential counts were performed by optical microscopy, on slides stained using the panoptic method.
