*4.2. Animals*

Six-week-old male Wistar rats (180–200 g) were obtained from an animal facility. The rats were housed in plastic cages at room temperature (25 ◦C) and humidity of 10% along with 12:12 light–dark cycle. The rats were given free access to water and food. The study was approved by the ethical committee. The study protocol was approved by the Institutional Review Board (No. RAKMHSU-REC-08-2019-F-P).

#### *4.3. LPS-Induced Endotoxemia and Survival Study*

The rats were divided into four groups (n = 10). The control group was treated with normal saline; Group II received an intraperitoneal (i.p) injection (10 mg/kg) of LPS as disease control. Rats in Group III were treated with ZIN 150 mg/kg (p.o) 2 h before LPS challenge. Animals in Group IV were treated with ZIN 150 mg/kg p.o only. Survival of the rats was monitored up to 96 h post LPS treatment.
