3.3.4. Antioxidant Capacity

For antioxidant activity: the samples (1 g) were mixed with 10 mL of MeOH/water (80:20%, *v*/*v*) and with 1% of HCl, sonicated at 20 ◦C for 15 min and left for 24 h at 4 ◦C. Then the extract was again sonicated for 15 min, and centrifuged at 15,000g for 10 min. The ABTS and the FRAP assays were determined according to Re et al. [41] and Benzie and Strain [42], respectively, by Synergy H1 spectrophotometer (BioTek Instruments Inc., Winnoski, VT, USA). The antioxidant capacity was expressed as mmol of Trolox per 100 g d.m.
