*4.5. Cell Apoptosis Analysis*

Following treatment with different concentrations of multifloroside for 48 h, the apoptosis of A431 cells was measured using the Annexin V-FITC/PI apoptosis detection kit according to the manufacturer's instructions. Apoptosis of the treated cells was then detected by flow cytometry (ACEA NovoCyte™, ACEA Biosciences, San Diego, CA, USA).
