*3.5. Total Anthocyanin Content*

The total anthocyanin contents (TAC) of the *H. acetosella* extracts were determined as described by Sutharut et al. [36]. The pH differential method, consisting of a KCl buffer (0.025 M, pH 1.0) and a CH3COONa buffer (0.4 M, pH 4.5), was used to determine the total anthocyanin content of the methanol extract prepared from each sample. A 1-mL aliquot of the extract was mixed with 4 mL of each of the buffers then incubated at 28 ◦C for 15 min so that the solution could equilibrate. The absorbance was measured at 510 nm and 700 nm using deionized water as a blank. The final result was converted to milligrams of cyanidin-3-glucoside equivalents (CGE) per gram dry weight (mg CGE/g dry weight).
