*4.10. Real-Time PCR Amplification*

All target transcripts were detected using quantitative real-time PCR (qPCR) and TaqMan assays. TaqMan gene expression assays (*OCLN*: Hs00170162\_m1, *CTNNB1*: Hs00355049\_m1, *MMP2*: Hs01548727\_m1, *MMP9*: Hs00234579\_m1, and *ERCC1*: Hs01012158\_m1) contained 20× mix of unlabeled PCR primers and TaqMan (Minor groove blinder (MGB)) probes (FAM dye-labeled). Glyceraldehyde-3-phosphate dehydrogenase (*GAPDH*) levels obtained using TaqMan control reagents (Hs02758991\_g1) were used as an endogenous control. PCR reactions were performed using an ABI Prism 7500 sequence detection system (Applied Biosystems) under previously described conditions [50].
