*3.10. Preparation of SW480-Conditioned Media*

To prepare conditioned media, SW480 cells were cultured on the 100 mm cell culture dish with the concentration of 5 × 10<sup>4</sup> cells/seeding. After 24 h, cells were washed with 1× PBS and changed to serum-free DMEM. Upon one day of incubation, media were collected and supernatant parts were filtered through 0.2 μm syringe filter for conditioned media (CM).

#### *3.11. C2C12 Myogenic Di*ff*erentiation in Cancer Conditioned Media*

To investigate the effect of PN on C2C12 myogenic differentiation under during cancer progression, conditioned media (CM) were applied as follows. First of all, C2C12 myoblasts were seeded onto six-well plates (4 × 10<sup>4</sup> cells/well) and cultured for 2 days in regular DMEM. After cells are attached, the mixture of CM and di fferentiation media (2% horse serum in DMEM) with the ratio of 1:3 were added and PN with the concentrations of 0.1, 0.5 and 1 μg/mL were treated.
