*2.5. Colony Formation*

As shown in Figure 3, DOX considerably affected the ability of cells to form colonies even when applied in subtoxic concentrations, unlike MAW and MAE extracts, which caused only a slight decrease in the colony-forming ability. However, combinations of DOX and MAE/MAW extracts were shown to lead to an even more pronounced decrease in the colony-forming ability of the treated cells compared to DOX treatment alone.

**Figure 3.** Effects of *M. aquifolium* extracts and DOX on A549 colony forming ability. (**A**) Representative images of colonies stained with Coomassie Brilliant Blue following treatment with DOX IC20, MAW (20 μg/mL), MAE (20 μg/mL), or their combinations. Experiments were performed at least three times. (**B**) Quantitative analysis of results presented in (A).
