*4.9. Statistical Analysis*

All measurements were made in triplicate, and all data are expressed as means ± SEM of three independent experiments. The significant differences from the respective control for each experimental group were examined by one-way analysis of variance (ANOVA) using GraphPad Prism 5 software. A value of *p* < 0.05 was considered to be statistically significant, and values of *p* < 0.01 or *p* < 0.001 indicated extremely significant differences.
