*2.2. Cell Cycle Analysis*

A549 cells treated with subtoxic concentrations of both MAE and MAW extracts for 24 h showed a slight increase in the percentage of cells in the G2/M phase of the cell cycle, but the changes induced by this treatment did not significantly differ from those in the controls. However, treatment of cells with IC20 and IC50 DOX led to a significant increase in the percentage of cells in the G2/M phase (Figure 1A), while cells treated with combination of DOX (IC20 or IC50) and MAW or MAE (20 μg/mL) were shown to accumulate in the G1 phase compared to those treated with DOX alone (Figure 1B). Samples treated with combinations of DOX (IC20 or IC50) and extracts showed an increase in the subG1 phase as well, compared to samples treated with DOX alone, and this increase in the number of subG1 cells was shown to be statistically significant for cells treated with combination of DOX and MAE (Figure 1B).
