*2.4. Cell Migration*

Both extracts demonstrated an improved ability to reduce cell migration compared to the control or DOX (Figure 2). After 48 h, this decrease was shown to be statistically significant in both extract-treated samples. Cells treated with DOX alone did not show any significant variation, while the migratory ability of cells treated with DOX and MAE or MAW was shown to be considerably lower after 48 h (Figure 2B).

**Figure 2.** Effects of *M. aquifolium* extracts and DOX on migration of A549 cells. The cells were treated with DOX IC20, MAW (20 μg/mL), MAE (20 μg/mL), or their combinations. (**A**) Representative images of one of three independent experiments. (**B**) Quantitative analysis of results presented in (A).
