*4.3. Nuclear Magnetic Resonance—NMR*

Nuclear Magnetic Resonance spectra were acquired on a Bruker *AVANCE III* 600 MHz spectrometer (Bruker Biospin, Karlsruhe, Germany) equipped with a 5 mm Triple Resonance Broadband Inverse (TBI) probe at 25 ◦C. 1H NMR (1D, 600.173 MHz) spectra were acquired using a f1 pre-saturation sequence with 32 transient scanning, 64 k data points, and 13 kHz bandwidth. 13C NMR spectra were obtained using the 1D sequence with power decoupling. Isolated HSD was dissolved in DMSO-*d6* (20 mg mL−1). Two-dimensional spectra (Heteronuclear Single Quantum Coherence—HSQC) were acquired using the 600.17 MHz frequency domain spectrometer F2 and 150.91 MHz in the F1 domain with a free induction decay size (FID) of 4096 (F2) and 256 (F1) data points and 16 "ghost" scans were used. HSQC (*hsqcedetgpsp.*) spectra were recorded with an acquisition time of 2.129 × 10−<sup>1</sup> s (F2) and 4.437 × 10−<sup>3</sup> s (F1) pulse sequence. The Heteronuclear Multiple Bond Correlations (HMBC) were acquired using the 600.17 MHz frequency domain spectrometer F2 and 150.91 MHz in the F1 domain with a free induction decay size (FID) of 2048 (F2) and 256 (F1) data points and 16 "ghost" scans were used. HMBC spectra were acquired with an acquisition time of 2.130 × 10−<sup>1</sup> s (F2) and 4.437 × 10−<sup>3</sup> s (F1), using the *hmbcgplpndgf*. pulse sequence. The spectra were processed in MestreNova software, using LB = 1.00 for F2 and LB = 0.30 for F1.
