*4.6. Chelation Properties of Hesperidin*

Chelation properties of HSD were tested through the interaction of HSD with copper(II), following the procedure in Selvaraj et al. [58]. For the experiment, HSD (1 mmol <sup>L</sup>−1) was dissolved in 50 mL of methanol, mixed with 0.199 g of copper(II) acetate in 25 mL of double distilled water and stirred for 6 h at room temperature. The pale green insoluble precipitate was obtained and filtered and washed with water and methanol to remove the excess of HSD and copper(II) acetate. The product was then air-dried. The greenish precipitate obtained was vacuum dried and characterized.
