*3.2. Cell Culture and Treatment*

The human CRC cell-lines, SW480, HCT116, HT29 and WiDR were purchased from the American Type Culture Collection (Rockville, MD, USA). G12V KRAS mutated SW480 (KRASG12V), G13D KRAS mutated HCT116 (KRASG13D), KRAS wild types of HT29 and WiDR were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS; Invitrogen), and 1% penicillin-streptomycin (P/S; Sigma, St. Louis, MO, USA). C2C12 cells were purchased from ATCC and cultured in DMEM (Dulbecco's modified Eagle's medium) containing 10% FBS and 1% P/S in a 5% CO2 atmosphere at 37 ◦C.
