*2.5. DPPH Assay*

The methanolic solution of DPPH radicals (1 cm3) prepared to the concentration of 3 × 10−<sup>4</sup> mol dm−<sup>3</sup> was added to 2.5 cm<sup>3</sup> of the analyzed extracts [30]. Furthermore, the blank solution composited of 1 cm<sup>3</sup> of methanol and 2.5 cm<sup>3</sup> of the extracts, as well as the control solution composited of 1 cm<sup>3</sup> of DPPH radicals and 2.5 cm<sup>3</sup> of methanol were prepared. Absorbance was measured at 517 nm in relation to methanol after incubation for 30 min at room temperature. The inhibition of DPPH radicals (%) was calculated as follows (Equation (1)):

$$I\_{\rm DPPH}(\%) = \frac{A\_{\rm c} - (A\_{\rm s} - A\_{\rm B})}{A\_{\rm c}} \times 100\tag{1}$$

where, *IDPPH* is the inhibition of DPPH radicals expressed in %, *As* is the absorbance of the samples treated with DPPH solution, *AB* is the absorbance of the blank, and *AC* is the absorbance of control.
