2.2.3. Microbiological Analysis

Microbiological growth of total vial count (TVC), total coliform count (TCC), *E. Coli, S. Aureus,* and *L. monocytogenes* was determined on days 0, 4, 7 and 11 from elaboration. Mass seeding was performed on Rapid E. Coli (to determine *E. Coli* and TCC), PCA (TVC), and Rapid L. mono (*L. monocytogenes*). All media were sterilised at 121 ◦C for 20 min according to product indications. Peptone water (OXOID, Ltd. CM0087 Basingstoke, Hampshire, United Kingdom) was used to make the dilutions. A laminar flow hood (Telstar, BIO-II-A, Spain) was used to carry out the analysis. Finally, plates were incubated for 24 h at 45 ◦C for *E. Coli*, 24 h at 37 ◦C for TCC, 48 h at 37 ◦C for TVC, and 48 h at 37 ◦C for *L. monocytogenes*. Results were obtained by triplicated and expressed in cfu/g.
