**5. Conclusions**

The MAE technique increased the yield of polyphenol compounds and bixin extracted from the annatto seeds, reducing energy consumption and increasing the antioxidant capability of extracts obtained. Despite the fact that the active compounds had a larger yield as compared to leaching, the antimicrobial activity did not present significant differences.

**Author Contributions:** Conceptualization, J.Q.Q., G.C.G., and J.R.C.; Investigation, J.Q.Q., A.C.T., L.M.R., and M.S.G.; writing—Original draft preparation, J.Q.Q., A.C.T., L.M.R., and M.S.G.; Writing—Review and editing, G.C.G. and J.R.C.; Supervision, G.C.G., and J.R.C.; funding acquisition, J.Q.Q., G.C.G., and J.R.C.

**Funding:** This work was sponsored by Colciencias through the gran<sup>t</sup> 727 of 2015 for the formation of Colombian PhDs and 715 for research and development projects in engineering-2015. The authors also thank the sustainability strategy of CODI 2018-2019.

**Conflicts of Interest:** The authors declare no conflict of interest.
