*2.5. Cell Line*

The HMC-1 cell line was kindly provided by Dr. Joseph H. Butterfield (Mayo Clinic, Rochester, NY, USA) and cultured in Iscove's Modified Dulbecco's Medium (IMDM- Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Cultilab, Campinas, SP, Brazil), 40 U/mL penicillin/streptomycin (Sigma, St. Louis, MS, USA) and 1.2 mM α-thioglycerol (Sigma, St. Louis, MS, USA). Cells were maintained at 37 ◦C in a humidified incubator at 5% CO2. Culture media was exchanged every 3–4 days with splitting of cultures at a confluency of 80–90%.
