*2.6. IFN*β *Promoter Reporter Assay*

HEK293 cells seeded in 96-well plates were transiently transfected with 50 ng of ZIKV-NS3 or -NS5 expression plasmid, 20 ng of a plasmid encoding firefly luciferase (F-Luc) under the control of the *IFN*β promoter and 5 ng pRL-TK, a plasmid which constitutively expresses renilla luciferase (R-Luc). Twenty-four hours later, cells were transfected with 5 ng IVT–RNA or 50 ng Hela–EMCV–RNA per well [13]. F-Luc activity was determined 24 h after RNA transfection using Dual-Luciferase Reporter Assay System (Promega) and normalized to R-Luc activity.
