*2.3. Western Blotting (WB)*

Cell lysates were performed in Radioimmunoprecipitation assay buffer (RIPA buffer). All subsequent steps of immunoblotting followed previous descriptions [22,23]. Primary antibodies were used at 1:1000 dilutions. Anti-rabbit immunoglobulin-horseradish peroxidase and anti-mouse immunoglobulin-horseradish peroxidase conjugates were used as secondary antibody (dilution 1:2000). Blots were revealed with enhanced chemiluminescence (ECL) detection reagents using an Amersham Imager 680 (GE, Buc, France).
