*2.10. Statistical Analysis*

The data derived from control and ZIKV-infected placentae obtained from the relative mean fluorescence intensity images and Western blot densitometry analysis were compared for statistically significant differences using the Mann-Whitney *U* test. The variances obtained with claudin-4 results were equal among the two groups; therefore, we applied a Student's *t*-test to include effect size estimations; since both groups had similar standard deviations, we used Cohen's *d*. For the Western blots of occludin, the data were further analyzed to include effect size estimation with Glass'delta, since each group had a different standard deviation. The Graphs were generated with GraphPad Prism 5.01 software (GraphPad Prism 5.01, La Jolla, CA, USA, 2007).
