*2.4. Immunohistochemistry*

The protocol for immunohistochemistry was described previously by our group [25]. Briefly, the slides were incubated overnight at 4 ◦C with a 1:200 dilution of the mouse monoclonal antibody IgG antibody against Zika NS1 (Arigo Biolaboratories, Taiwan, Republic of China). Then, sections were maintained with a rabbit anti-mouse IgG conjugated to horseradish peroxidase (Spring Bioscience Corporation, CA, USA) for 40 min at room temperature. We visualized the sections by light microscopy (Olympus, Tokyo, Japan), and digital images were obtained using Image-Pro Plus software version 7.0.
