*2.8. qRT-PCR*

Cells were lysed and total RNA was extracted using the QIAshredder (Qiagen) and RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA was reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen) into cDNA that was then used for qPCR with either TaqMan Universal PCR Master Mix (Applied Biosystems) or SYBR green PCR kit (Life Technologies). *CT* values were normalized to GAPDH (Δ*CT*). TaqMan primer probes used include GAPDH (Assay ID: Hs02758991 g1), IFIT1 (Assay ID: Hs03027069 s1) and MX1 (Assay ID: Hs00895608 m1). SYBR green primer probes used include GAPDH (fwd: CATGGCCTTCCGTGTTCCTA, rev: CCTGCTTCACCACCTTCTTGA) and ZIKV (fwd: CGAGGAACATCCAGACTC, rev: ATTGGAGATCCTGAAGTTCC).
