**2. Materials and Methods**

#### *2.1. Viruses, Cell Lines, Antibodies and Reagents*

The clinical isolate PF-25013-18 (PF13) and the molecular clones of ZIKV (BR15MC and MR766MC) have been previously described [20,21]. Vero cells (ATCC, CCL-81) and HEK 293T (CRL-3216) were cultured at 37 ◦C under a 5% CO2 atmosphere in MEM medium (PAN Biotech, Aidenbach, Germany), supplemented with 5% or 10% heat-inactivated fetal bovine serum (FBS) (PAN Biotech, Aidenbach, Germany). A549-Dual ™ cells (InvivoGen, Toulouse, France, a549d-nfis) designated hereafter as A549 cells were maintained in MEM medium supplemented with 10% heat-inactivated FBS. A549 cells were maintained in growth medium supplemented with 10 μg.mL−<sup>1</sup> blasticidin and 100 μg.mL−<sup>1</sup> zeocin (InvivoGen, Toulouse, France).

To detect the ZIKV envelope E protein (ZIKV-E), we used the mouse anti-pan flavivirus envelope E protein mAb 4G2 produced by RD Biotech or the rabbit anti-ZIKV-E-DIII that was kindly provided by Dr. Valerie Choumet (Institut Pasteur). The rabbit anti-BAX (#2772) and anti-active caspase 3 (#9664) antibodies and the mouse anti-cytc 6H2.B4 (#12963) were purchased from Cell Signalling Technology (Ozyme, Saint-Cyr-l'École, France). The mouse anti-BAX clone 2D2 was from Invitrogen (Thermofisher, Les Ulis, France). The anti-mitochondria antibody MTC02-3298 was from Abcam (Cambridge, UK). Donkey anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 IgG antibodies were from Invitrogen (Thermofisher, Les Ulis, France). Horseradish peroxidase-conjugated anti-rabbit (ab97051) and anti-mouse (ab6789) antibodies were purchased from Abcam (Cambridge, UK).

All reagents were from Sigma Aldrich (Humeau, La Chapelle-Sur-Erdre, France) except when indicated. ABT-737 (ab141336) was purchased from Abcam (Cambridge, UK).
