*3.4. Gene Ontology*

Gene ontology (GO) enrichment analysis of the Merged ZIKV PIN identified a subset of proteins mainly involved in 13 non-redundant biological processes (Figure S1). Among the hits identified, 13 are members of the proteasome complex (11 unique to the 26S subunit) and five members of the chaperonin-containing TCP1 (CCT) complexes (8.7% and 3.3% of our PIN, respectively) (Figure S1 and Table S5). GO enrichment analysis of cellular components revealed an enrichment of peptidase complex, chaperone complex, and ficolin-1-rich granules (Table S6).

Next, we applied unsupervised clustering of bait sets according to their GO enrichment ratios for biological processes and cellular components (Figure 3B,C and Tables S7 and S8). Interestingly, protein bait sets were clustered into two major groups (NS1, NS2A and NS3 versus NS2B, NS4A, NS4B, and NS5).
