*2.3. Immunofluorescence Assay*

Immunofluorescence was performed as described in Rabelo et al., 2017 [25]. Antibodies were used at a dilution of 1:200 for a mouse monoclonal anti-Zika NS1 IgG (Arigo Biolaboratories, Taiwan, Republic of China), and a rabbit polyclonal antihuman c-Kit IgG (Santa Cruz, Texas, USA). After staining with primary antibodies, sections were incubated with an Alexa 488-conjugated rabbit anti-mouse IgG, Alexa 555-conjugated goa<sup>t</sup> anti-rabbit IgG, or Alexa 555-conjugated goa<sup>t</sup> anti-mouse IgG (ThermoFisher, Waltham, MA, USA). Slides were visualized by fluorescence microscopy (Olympus, Tokyo, Japan), and digital images were obtained using Image-Pro Plus software version 7.0.
