*2.6. Immunofluorescence of Clinical Samples*

Tissues were fixed in 4% PFA, embedded in OCT media (ThermoFisher, Waltham, MA, USA), and stored at −80 ◦C until sectioned into 10 μm cryosections using a Leica CM3050-S cryostat (Leica Biosystems, Buffalo Grove, IL, USA), and sections were stored at −80 ◦C until immunostaining. Sections were immunostained for ZIKV using a mouse-anti-ZIKV Envelope (E) protein primary antibody (FL0006, Kerafast, Boston, MA, USA), and visualized with a donkey-anti-mouse-AlexaFluor 488 secondary antibody (ab150101, Abcam, Cambridge, MA, USA). Sensory neurons were immunostained with anti-PGP9.5 (NB300-675, Novus Biologicals, Centennial, CO, USA) and satellite glial cells with anti-glutamine synthetase (ab73593, Abcam, Cambridge MA, USA) antibodies, followed by species-specific secondary antibodies conjugated to AlexaFluor 488 (Fisher Scientific, Hamptom, NH, USA). CNS neurons were immunostained with anti-NeuN conjugated to AlexaFluor 488 (ABN78A4, EMD Millipore, Burlington, MA, USA). Slides were treated with SlowFade Gold Antifade Mountant (ThermoFisher, Waltham, MA, USA) before cover slipping. Slides were visualized and imaged using an IX71 inverted fluorescence microscope (Olympus Life Sciences, Waltham, MA, USA).
