*2.11. Transmission Electron Microscopy Procedure*

HMC-1 cells were infected with ZIKV at a MOI of 1 for 30 min or 24 h and then fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate bu ffer (pH 7.2). Cells were post-fixed with 1% bu ffered

osmium tetroxide, dehydrated in an acetone series (30, 50, 70, 90, and 100%) and then embedded in EPON (Electron Microscopy Sciences, Hatfield, PA, USA) through polymerization at 60 ◦C for 3 days. Ultrathin sections (60–90 nm) were contrasted with uranyl acetate and lead citrate before visualization using a JEOL 1001 transmission electron microscope (Jeol Ltd., Tokyo, Japan).
