*2.10. ELISA Assays*

The quantity of cytokines and factors released from mast cells by infection with ZIKV was measured by ELISA. Supernatants from HMC-1 cells infected at a MOI of 1 for 30 min, 6 h or 24 h were evaluated for IL-6 (900-T16), IL-10 (900-K21), TNFα (900-T25) and VEGF (900-K10) with commercial ELISA assay kits (Peprotech Inc. Rocky Hill, NJ, USA), according to the manufacturer's instructions.
