*2.10. Quantification of Peroxisomes*

*Z*-stack images acquired using a confocal microscope were exported from Volocity 6.2.1 as an OEM.tiff file. The exported images were then processed using Imaris 7.2.3 software (Bitplane, Concord, MA, USA). Peroxisomes within polygonal areas that excluded the nucleus were quantified (quality and voxel). Within the selected regions, the absolute intensity of the peroxisomes was determined and then entered into a Microsoft Excel spreadsheet. The data were then analyzed using student's *t*-test. In each cell, peroxisomes were selected based on the absolute pixel intensity in the corresponding channel, and their numbers were then determined. Only those SKL/PMP70-positive structures with volumes between 0.001 and 0.05 μm<sup>3</sup> were included for measurement.
