*2.3. ZIKV*

The Brazilian ZIKV isolate ZIKV/*H. sapiens*/Brazil/PE243/2015 was originally described in [24] and was grown on Vero cells. Viral titers were determined by plaque assay on A549 BVDV NPro cells. These cells are optimized for virus growth as they stably express the NPro protein of bovine viral diarrhea virus (BVDV), which induces degradation of IRF3 [25].
