*2.6. Immunoblot Assay*

Cell lysates were performed in RIPA lysis buffer or buffer A (cell fractionation). All subsequent steps of immunoblotting were performed as previously described [32,33]. Primary antibodies were used at 1:500 dilutions. Anti-mouse immunoglobulin-horseradish peroxidase and anti-rat immunoglobulin-horseradish peroxidase conjugates were used as secondary antibodies (dilution 1:2000). Blots were revealed with ECL detection reagents (Amersham, Little Chalfont, United Kingdom).
