*2.10. Protein Stability Assay*

HEK293FT cells were transfected with expression vectors containing GST-tagged PIAS1 and GFPor CBP-tagged NS5 (empty expression vectors were used as negative controls) and treated 24 h later with 10 μg/mL of cyclohexamide (or Dimethylsulfoxide) for varying lengths of time. Whole cell lysates were analyzed by Western blotting using anti-GST, anti-CBP, or anti-GFP and anti-β-actin (Santa Cruz Biotechnology; C4).
