*2.7. ZIKV Infections*

Infections were performed by varying the multiplicity of infection (MOI) at 0.1, 0.2 and 1.0. ZIKV viral particles per host cell. Virus was added to cell culture and incubated for 1 h at 37 ◦C prior to removal of unattached viral particles and a further incubation of 6 h or 24 h. For a 30 min time point, virus was incubated with cells for 30 min before rinsing and preparation of flow cytometry. As a negative control, cells were incubated in the same conditions with a mock viral stock consisting of a supernatant of non-infected Vero cells.
