*2.3. Virus Strains*

Flaviviruses WNV-TX, WNV-MAD, ZIKV MR766, ZIKV FSS13025, ZIKV Fortaleza, JEV Nakayama, DENV-2 New Guinea C, and DENV-4 H241 were grown and titred on Vero cell monolayers in cDMEM. All infections were initiated using a low volume inoculum of virus in complete media (media dependent on cell type) incubated at 37 ◦C for 2 h with rocking. Inoculum was subsequently removed and replaced with fresh complete media. The encephalomyocarditis virus (EMCV) strain Mengo infectious clone was a kind gift from Ann C. Palmenberg (UW-Madison). Virus stocks were grown and titred on Vero cell monolayers in cDMEM. Sendai virus (SeV) Cantell strain was sourced from Charles River Laboratories (USA). All virus stocks were confirmed to be free of mycoplasma contamination.
