*4.1. Cell Culture*

The uveal melanoma cell line OMM-1 [42] was provided by Dr. Sarah Coupland. RPMI 1640 (Merck, Darmstadt, Germany) was used for cultivation (with 10% fetal calf serum (Linaris GmbH, Wertheim-Bettingen, Germany) and 1% penicillin/streptomycin (Merck, Darmstadt, Germany)).

The human RPE cell line ARPE-19 [43] was purchased from ATCC. The cultivation medium was HyClone DMEM (GE Healthcare, München, Germany) with 1% penicillin/streptomycin, 1.2% HEPES, 1% non-essential amino acids (all from Merck, Darmstadt, Germany), and 10% fetal calf serum, as previously described [11].

Primary porcine RPE cells were prepared as described before [30,44]. RPE cells were detached from porcine eyes by trypsin incubation and cultivated in HyClone DMEM (GE Healthcare, München, Germany) supplemented with penicillin/streptomycin (1%), HEPES (2.5%), non-essential amino acids (1%) (all Merck, Darmstadt, Germany), and 10% fetal calf serum.

ARPE-19 and RPE were treated at confluence, and OMM-1 cells were treated at subconfluence.
