*3.5. Statistical Analysis*

All data were analyzed by one-way ANOVA, followed by the Tukey test for multiple comparisons. A *p*-value less than 0.05 was considered statistically significant. All analyses were performed using the GraphPad Prism 8.00 for Windows software (GraphPad Software, San Diego, CA, USA).

### *3.6. Characterization of Colloid Nanoparticles*

After purification by HPLC, samples were prepared in 1 mL of Millipore water at 0.1 mM of each compound **1**–**3** and mixtures **2** and **3**. After sonication for 30 min at 35 ◦C, the solutions were maintained at room temperature (20 ◦C) for 24 h. The mean di ffusion coe fficients were obtained as an average of at least three measurements at 25 ◦C.
