*4.6. Oxidative Stress*

To induce oxidative-stress-related cell death, OMM-1 cells were treated with 1 mM H2O2 and ARPE-19 cells were treated with 500 μM TBHP as previously established [11,16] to reduce cell viability to nearly 50% (tested via MTS assay, see below). Before insult with the oxidative agents, cells were incubated with 1, 10, 50, and 100 μg/mL LH extracts.

### *4.7. Methyl Thiazolyl Tetrazolium (MTT) Assay*

The widely used MTT assay [45] was conducted as previously described [4,11] and was used after taking supernatants for the VEGF ELISA (see below) and after 24 h sulfated fucan (1–100 μg/mL) stimulation. The cells were incubated with 0.5 mg/mLMTT for 2 h and dissolved in DMSO.Measurement was taken at 550 nm with an Elx800 (BioTek Instruments Inc., Bad Friedrichshall, Germany).
