*4.3. Monosaccharide Analysis*

The sulfated fucan samples were hydrolyzed using 3 mol/L trifluoroacetic acid (TFA) at 95 ◦C for 12 h. The hydrolyzed monosaccharides were fractionated and analyzed through ion-exchange chromatography equipped with a CarboPac PA20 (Thermo Fisher Scientific Inc., Waltham, MA, USA) column.
