*4.5. Molecular Weight Determination*

The molecular weight of the sulfated fucan was determined by size-exclusion chromatography (SEC) in the form of HPLC equipped with online multiangle static light scattering (MALS). The measurements were performed at ambient temperature using a Shodex LB-806 and a 2500 PWXL column as a separator. The measurement was executed with a Dawn HELEOS-8+ multiangle laser light scattering photometer (Wyatt, Santa Barbara, CA, USA) (λ0 = 660 nm) and a subsequent Optilab T-rEX di fferential refractometer. The mobile phase was 0.10 mol/L Na2HPO4 (pH = 7), and the flow rate was 0.2 mL/min. The injection volumes were 50 μL and 100 μL, with 1 g/mL. The data were obtained and processed using Astra (v. 7) software (Wyatt, Santa Barbara, CA, USA).
