**Antioxidant and Cytotoxic E**ff**ects on Tumor Cells of Exopolysaccharides from** *Tetraselmis suecica* **(Kylin) Butcher Grown Under Autotrophic and Heterotrophic Conditions**

**Geovanna Parra-Riofrío 1,2,\*, Jorge García-Márquez 3, Virginia Casas-Arrojo 4, Eduardo Uribe-Tapia 2 and Roberto Teófilo Abdala-Díaz 4,\***


Received: 27 September 2020; Accepted: 25 October 2020; Published: 26 October 2020

**Abstract:** Marine microalgae produce extracellular metabolites such as exopolysaccharides (EPS) with potentially beneficial biological applications to human health, especially antioxidant and antitumor properties, which can be increased with changes in crop trophic conditions. This study aimed to develop the autotrophic and heterotrophic culture of *Tetraselmis suecica* (Kylin) Butcher in order to increase EPS production and to characterize its antioxidant activity and cytotoxic effects on tumor cells. The adaptation of autotrophic to heterotrophic culture was carried out by progressively reducing the photoperiod and adding glucose. EPS extraction and purification were performed. EPS were characterized by Fourier-transform infrared spectroscopy and gas chromatography-mass spectrometry. The antioxidant capacity of EPS was analyzed by the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method, and the antitumor capacity was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, showing high activity on human leukemia, breast and lung cancer cell lines. Although total EPS showed no cytotoxicity, acidic EPS showed cytotoxicity over the gingival fibroblasts cell line. Heterotrophic culture has advantages over autotrophic, such as increasing EPS yield, higher antioxidant capacity of the EPS and, to the best of our knowledge, this is the first probe that *T. suecica* EPS have cytotoxic effects on tumor cells; therefore, they could offer greater advantages as possible natural nutraceuticals for the pharmaceutical industry.

**Keywords:** *Tetraselmis suecica*; autotrophic culture; heterotrophic culture; exopolysaccharides; antioxidant capacity; cytotoxic effects on tumor cells
