*3.3. Genome Assembling*

Raw nanopore data was basecalled using Guppy v3.2.2 (Oxford Nanopore Technologies, Oxford, UK). After quality filtering using NanoFilt [50] and residual adapter removal using Porechop (https://github.com/rrwick/Porechop), the obtained dataset was quality checked using NanoPlot [50]. Long nanopore reads were then assembled using Flye v2.6 [51]. Flye assembled contigs were further polished using Illumina sequencing reads and Unicycler\_polish pipeline (https://github.com/rrwick/Unicycler).
