*3.4. Genome and NRPS Alignment*

Genome assembly was annotated using the NCBI Prokaryotic Genome Annotation Pipeline [52] with the assistance of prokka [53] refine annotation, with additionally curated database comprised of sequences selected by Nostocales order from NCBI non-redundant and refseq\_genomes (280 positions) databases, enriched by 35 NRPS/PKS clusters selected by cyanobacteria phylum. To create circular maps of *N*. *edaphicum* CCNP1411 genome, the CGView Comparison Tool [54] was engaged with additional GC skew and GC content analyses.

Selected span for potential NRPS cluster was confirmed with BLASTn, BLASTp [55] and antiSMASH [56]. ORFs start codons within a putative cluster were verified by the presence of ribosome binding sites, 4–12 nucleotides upstream of the start codon. Schematic comparison of ORF BLASTn from relative synthetases, AY167420.1 and CP026681.1, was visualized by EasyFig program (http://mjsull.github.io/Easyfig/files.html). Annotated regions of NRPS span were subjected for NCBI Conserved Domain Database search [57] with a set e-value threshold (10−3), determining evolutionarily-conserved protein domains and motifs against CDD v3.18 database. Recognized motifs were selected using samtools v.1.9 and were subjected for protein structure and function prediction by I-TASSER [58], and results were confirmed with literature reports, PKS/NRPS Analysis Web-site prediction [59] and reevaluated using MEGAX suite [60]. Amino-acid sequence was visualized by BOXSHADE 3.2 program (https://embnet.vital-it.ch/software/BOX\_form.html). Determination of domain ligand binding and active sites was achieved using COFACTOR and COACH part of I-TASSER analyses confirmed by MUSCLE amino acid alignment from MEGA X.

## *3.5. Data Deposition*

Genomic sequences generated and analyzed in this study were deposited in the GenBank database under BioProject number: PRJNA638531.
