*3.6. PTP1B Inhibition Assays*

The PTP1B Inhibition assay was performed in 96-well plates [25]. The compound (10 μL) was added to the 99-μL reaction buffer solution, which consisted of 10 mM Tris (pH 7.4), 50 mM NaCl, 2 mM dithiothreitol (DTT), 1 mM MnCl2, and 10 mM para-nitrophenyl phosphate (*p*NPP). The reaction mix was pre-warmed using a block heater at 37 ◦C. The recombinant PTP1B solution (1 mg/mL, 1 μL) was mixed in each well. An NaOH solution (10 μL, 0.1 M) was added to stop the reaction. The absorbance was recorded at 405 nm using a microplate. Sodium vanadate was used as a positive control.
