*4.2. Microorganism Material and Culture*

*Streptomyces qinglanensis* 172205 was isolated from a mangrove soil sample and identified as a novel species [13]. This strain was cultured on ISP2 agar plates at 28 ◦C. *Escherichia coli* Top 10 was used for cloning and *E. coli* ET12567/pUZ8002 was used for intergeneric conjugation, which cultured on LB agar plates at 37 ◦C.
