*3.10. RAW 264.7 Cell Culture*

A RAW 264.7 murine macrophage cell line was purchased from American Type Cell Culture (Rockville, MD, USA). Cells were cultured in DMEM with 10% fetal bovine serum and antibiotic−antimycotics (i.e., penicillin G sodium, 100 units/mL; streptomycin, 100 μg/mL; amphotericin B, 250 ng/mL) at 37 ◦C in a humidified incubator with 5% carbon dioxide. All reagents used for cell culture were purchased from Gibco® Invitrogen Corp. (Grand Island, NY, USA).
