*2.5. Data Extraction*

Data were extracted from studies included in this review by two independent reviewers (A.W. and T.B.) using an electronic form developed by the authors (see Supplementary Table S4 for full extraction templates). Extracted data included specific details about the mice populations sampled, the study design, blood sample routes, and the outcomes of significance to the review objective, being an indicator of animal well-being. If the data were presented as figure or other form, they were extracted with the Software Getdata Graph Digitizer 2.26 0.20 (S. Federov, Moscow, Russia). Any disagreements that arose were resolved through discussion. Authors of papers were contacted to request missing or additional data, where required.
