**5. Conclusions**

In conclusion, this small study has shown CBE can be an ideal and humane method compared to other techniques (i.e., BFT) when uncontaminated tissues are needed. Thus far, it has been shown to have favorable experimental, animal and human welfare outcomes in guinea pigs where high quality, uncontaminated non-brain tissue collection is required. Future research could increase the sample size

and use alternative parameters (e.g., AEP, EEG, post-mortem and histological changes) to corroborate the evidence for the e ffectiveness of CBE. Other areas to be explored could be the use of an industrial CB, alternative restraining methods or devices, and the inclusion of formal surveys to evaluate operator attitudes and experiences in performing CBE.

**Author Contributions:** Conceptualization, S.C. and J.H.; methodology, S.C. and J.H.; formal analysis, S.C.; investigation, S.C.; resources, S.C.; data curation, S.C.; validation, S.C.; writing—original draft preparation, M.K. and S.C.; writing—review and editing, S.C. and J.H.; visualization, S.C. and M.K.; supervision, S.C.; project administration, S.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Acknowledgments:** This work was supported by the University of Melbourne (UoM) Animal Welfare Team in the O ffice of Research Ethics and Integrity. The authors would like to thank the UoM Bioresources Platform facility sta ff, Maya Kesar and Keith Anderson. We would also like to acknowledge Gary Anderson and Andrew Jarnicki, from the Mazzone Laboratory of Respiratory Sensory Neuroscience from the UoM Department of Pharmacology and Therapeutics, as this work could not have been completed without their support.

**Conflicts of Interest:** The authors declare no conflict of interest.
